High throughput equipment for generating next generation sequencing libraries

用于生成下一代测序文库的高通量设备

• 批准号: RTI-2021-00536
• 负责人: Zayed, Amro
• 金额: $ 7.46万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=717195
• 关键词: throughput; equipment; generating; next; generation

Next generation sequencing (NGS) has revolutionized biology by allowing researchers to rapidly characterize genomes and genetic diversity, gene expression and epigenetic modifications, and even the complex assemblages of interacting organisms found within host species and entire ecosystems. Here, we request a suite of high-throughput equipment needed to undertake three important steps in NGS tissue lysis prior to nucleic acid extraction, sonication to generate appropriately sized DNA or RNA fragments for constructing sequence libraries, and quality control assessment of nucleic acids and sequencing libraries. This equipment is urgently needed to create capacity in NGS for a growing number of researchers, including several early career researchers, to train a large cohort of diverse HQP on in-demand research techniques (thereby providing considerable employment opportunities), and to vastly increase the cost and time efficiency of the research programs of seven faculty.

下一代测序（NGS）使研究人员能够快速表征基因组和遗传多样性，基因表达和表观遗传修饰，甚至宿主物种和整个生态系统中相互作用的生物体的复杂组合，从而彻底改变了生物学。在这里，我们需要一套高通量设备，用于在核酸提取之前进行NGS组织裂解的三个重要步骤，超声处理以产生适当大小的DNA或RNA片段用于构建序列库，以及核酸和测序库的质量控制评估。 这种设备是迫切需要在NGS为越来越多的研究人员，包括几个早期的职业研究人员创造能力，以培训大量的不同的HQP在需求的研究技术（从而提供相当多的就业机会），并大大增加了七个教师的研究计划的成本和时间效率。