Antimicrobial resistance and improved detection of Tenacibaculum in B.C. Atlantic salmon

BC 省的抗菌素耐药性和粘杆菌检测的改进

• 批准号: 561445-2020
• 负责人: Russell, Spencer
• 金额: $ 2.18万
• 依托单位: Vancouver Island University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=719039
• 关键词: Antimicrobial; resistance; improved; detection; Tenacibaculum

Canadian finfish aquaculture is a significant driver of the BC economy employing thousands of individuals along the BC coast. Mowi Canada West (MCW), the largest producer of farmed Atlantic salmon in BC, loses many thousands of marketable fish to "tenacibaculosis" caused by an infection with multiple species of the bacteria Tenacibaculum. Costs for antibiotic treatment contribute to additional financial losses which compounds the impact of Tenacibaculum infections. A current theory related to the underlying mechanism of tenacibaculosis is the disruption of bacterial communities (dysbiosis) found on fish and the surrounding netpen environment following the transfer of salmon smolts from freshwater hatcheries to saltwater growout net pens. Next Generation Sequencing (NGS) offers the ability to both identify and quantify the bacterial community on fish and within water surrounding net pen sites. This project will build on a strong partnership between University of Guelph (UGuelph) Vancouver Island University (VIU) and MCW established through NSERC Engage, Engage Plus grants, and several local grants bringing VIU researchers together with local companies and the BC Center for Aquatic Health Sciences (CAHS). The objectives of this project are to 1) collect and screen Tenacibaculum Isolates from MCW BC net pen sites for minimum inhibitory concentrations to 11 veterinary antibiotics used in aquaculture; 2) produce full genomic comparison of the select Tenacibaculum isolates including virulent factors, antimicrobial resistance genes and phylogenomic analysis using the minION sequencing platform; 3) develop a target gene high-throughput assay to investigate Tenacibaculum species community distribution and dysbiosis using the Illumina MiSeq platform.; 4) assess the community distribution and relative abundance of Tenacibaculum species in salmon tissue samples taken pre-, during, and post-clinical outbreaks of tenacibaculosis experienced at MCW net pen sites in 2019-2020. This project will increase MCW's ability to manage disease presence, improve fish welfare, mitigate unnecessary antibiotic usage, and potentially identify key Tenacibaculum species for future vaccine development.

加拿大鳍鱼养殖业是不列颠哥伦比亚省经济的重要驱动力，在不列颠哥伦比亚省沿岸雇佣了数千名个人。加拿大西部Mowi （MCW）是不列颠哥伦比亚省最大的养殖大西洋鲑鱼生产商，由于多种链杆菌感染引起的“链杆菌病”而损失了数千条可销售的鱼。抗生素治疗的费用造成额外的经济损失，这加剧了腱杆菌感染的影响。目前有关韧杆菌病潜在机制的一个理论是，在鲑鱼幼崽从淡水孵化场转移到咸水生长网箱后，在鱼类和周围网箱环境中发现的细菌群落破坏（生态失调）。下一代测序（NGS）提供了识别和量化鱼身上和网笔周围水内细菌群落的能力。该项目将建立在圭尔夫大学（圭尔夫）温哥华岛大学（VIU）和MCW之间强有力的合作伙伴关系的基础上，该合作伙伴关系是通过NSERC Engage， Engage Plus赠款和几项当地赠款建立的，将VIU研究人员与当地公司和BC省水生健康科学中心（CAHS）聚集在一起。本项目的目的是：1)收集和筛选MCW BC网栏中分离的腱杆菌对11种水产养殖用兽用抗生素的最低抑菌浓度；2)利用minION测序平台对选定的Tenacibaculum分离株进行全基因组比较，包括毒力因子、耐药基因和系统基因组分析；3)利用Illumina MiSeq平台开发目的基因高通量检测方法，研究Tenacibaculum物种群落分布和生态失调；4)评估2019-2020年MCW渔场网笼鼠疫暴发前、期间和后采集的三文鱼组织样本中腱杆菌的群落分布和相对丰度。该项目将提高MCW管理疾病存在的能力，改善鱼类福利，减少不必要的抗生素使用，并可能为未来的疫苗开发确定关键的腱骨菌物种。