Evolution of mosquito-virus interactions: range expansions, viral coinfections and mosquito behaviour

蚊子-病毒相互作用的演变：范围扩大、病毒共感染和蚊子行为

• 批准号: RGPIN-2020-05200
• 负责人: Hunter, Fiona
• 金额: $ 2.91万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=740447
• 关键词: Evolution; mosquito; virus; interactions; range

There are three research foci in my lab. (1) TAXONOMY and SYSTEMATICS  We will be studying the incursions of two mosquito species into Canada, namely Aedes albopictus (Asian Tiger mosquito) and Aedes aegypti (Yellow Fever mosquito), by tracing the phylogenetic relationships of established and novel populations using DNA sequences. We will also undertake a revision of 8 species in the mosquito genus Culiseta to determine how they are related to one another; such information is necessary for understanding the transmission of Eastern Equine Encephalitis virus. (2) BEHAVIOURAL ECOLOGY  Earlier work on the effects of sugar-feeding among the blood-feeding Diptera has suggested that honeydew sugar meals offer enhanced flight capabilities and may protect mosquitoes from some protozoan infections. In contrast, nectar meals may offer enhanced vitellogenesis which presumably leads to increased egg production. We aim to examine the effects of honeydew vs. nectar meals on longevity and fecundity in mosquitoes. This will be followed by choice experiments in which we determine whether the gender, age, size, mating status and/or infection status of mosquitoes influences the type of sugar-meal ingested. (3) DISEASE TRANSMISSION In addition to ongoing vector competence studies to determine which mosquitoes are able to transmit which viruses, we are interested in the effects of dual infection with two strains of the same virus as well as with two different viruses. We intend to study Zika virus, Mayaro virus and eventually Dengue virus and Yellow Fever virus. Co-infection and superinfection studies will be done in cell culture first and then in live, infected mosquitoes in Brock's Containment Level 3 Lab with Insectary.

我的实验室有三个研究重点。 (1)分类学和昆虫学我们将研究两种蚊子入侵加拿大的情况，即白纹伊蚊（亚洲虎蚊）和埃及伊蚊（黄热病蚊），方法是利用DNA序列追踪已建立和新种群的系统发育关系。我们还将对库利塞蚊属的8种蚊子进行修订，以确定它们之间的关系;这些信息对于了解东方马脑炎病毒的传播是必要的。 (2)行为生态学早期关于吸血双翅目中糖喂食的影响的研究表明，蜜露糖餐提供了增强的飞行能力，并可能保护蚊子免受某些原生动物的感染。相比之下，花蜜餐可能会提供增强的卵黄发生，这可能会导致卵子产量增加。我们的目的是研究蜜露与花蜜对蚊子寿命和繁殖力的影响。随后将进行选择实验，确定蚊子的性别、年龄、大小、交配状态和/或感染状态是否会影响摄入的糖粉类型。 (3)疾病传播除了正在进行的媒介能力研究以确定哪些蚊子能够传播哪些病毒外，我们还对同一病毒的两种毒株以及两种不同病毒的双重感染的影响感兴趣。我们打算研究寨卡病毒、马亚罗病毒，最终研究登革热病毒和黄热病病毒。将首先在细胞培养物中进行共感染和重复感染研究，然后在Brock的3级昆虫实验室中进行活的受感染蚊子研究。