An Open Access Liquid Chromatography Mass Spectrometer for Sample Characterization

总结。我代表几位同事和整个UBC化学系，带头申请资金，为UBC化学质谱分析设施购买一台开放获取的液相色谱质谱仪（OA-LCMS），以取代一台已经使用了17年的破旧仪器。质谱（MS）是鉴定未知化合物和定量已知化合物的基本分析技术，因此对我们的培训任务至关重要。液相色谱-质谱联用技术在化学合成和药物开发等领域已成为一种强有力的化合物鉴定和纯度分析技术。现代LCMS仪器，连同自动软件使开放存取质谱（OAMS）成为可能。OAMS是指在没有MS设施人员干预的情况下，调查人员可以全天候使用的MS。它易于使用，化学家可以立即获得反应监测，使受训者能够随意工作，实时分析他们的样品。自助服务还为学员提供了最先进的分析机器的实践经验，并对产品特性有了更大的了解。更重要的是，设备的自助服务性质对于我们的学员了解如何将合成和/或未知化合物的色谱部分与质谱分析相关联至关重要。在培养学生表征复杂合成产品的过程中，本系的学员正在推动天然产物合成、方法开发、精细化学品/高级中间体催化以及新生物实体（包括多糖和糖肽）表征等方面的创新。此应用程序支持为UBC整个化学系服务的机器。我们培养了100多名从事有机、有机金属、无机和材料化学的化学家。这些受训者在工业和教学中找到工作，一些pi已经从工业界获得了大量资金，而其他人则参与了公司的创建。这台仪器将由化学系提供实物支持，它对我们的培训任务至关重要，因为它支持研究企业各级化合物表征所需的基础设施。

Summary. On behalf of several colleagues and indeed the entire UBC Chemistry Department, I am spearheading a request for funds to purchase an open access liquid chromatography mass spectrometer (OA-LCMS) for the UBC Chemistry Mass Spectrometry Facility to replace a faltering instrument that is now 17 years old. Mass spectrometry (MS) is an essential analytical technique to identify unknown compounds and to quantify known compounds and thus is critical to our training mission. Liquid chromatography mass spectrometer (LCMS) has been established as a powerful analytical technique in compound identification and purity analysis in many fields including chemical synthesis and drug discovery. Modern LCMS instrumentation, along with walk-up software enables open access mass spectrometry (OAMS). OAMS refers to a MS that is available for use by investigators 24/7 without the intervention of MS facility personnel. It is easy-to-use and immediate accessible to chemists for reaction monitoring and it enables trainees to work at-will to analyze their samples in real-time. Walk-up service also gives trainees hand-on experience with state-of-the-art analytic machines and a greater appreciation for product characterization. More importantly, the self-service nature of the equipment is essential for our trainees to appreciate first-hand how to correlate chromatography fractions of synthetic and/or unknown compounds with MS analysis. In training students to characterize complex synthetic products, trainees in our department are driving innovation in terms of natural product synthesis, methodology development, catalysis of fine chemicals/advanced intermediates, and the characterization of new biological entities including polysaccharides and glycopeptides to name a few. This application supports a machine that serves the entire Chemistry Department of UBC. We train over 100 chemists working on organic, organometallic, inorganic, and materials chemistry. These trainees find jobs in industry and teaching and several PIs have received significant funding from industry while others are involved in company creation. This instrument, which will be supported by an in-kind contribution from the Chemistry department is critical to our training mission as it supports critically needed infrastructure to characterize compounds at all levels of the research enterprise.