An Open Access Liquid Chromatography Mass Spectrometer for Sample Characterization

用于样品表征的开放存取液相色谱质谱仪

• 批准号: RTI-2022-00618
• 负责人: Perrin, David
• 金额: $ 10.93万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=741829
• 关键词: Open; Access; Liquid; Chromatography; Mass

Summary. On behalf of several colleagues and indeed the entire UBC Chemistry Department, I am spearheading a request for funds to purchase an open access liquid chromatography mass spectrometer (OA-LCMS) for the UBC Chemistry Mass Spectrometry Facility to replace a faltering instrument that is now 17 years old. Mass spectrometry (MS) is an essential analytical technique to identify unknown compounds and to quantify known compounds and thus is critical to our training mission. Liquid chromatography mass spectrometer (LCMS) has been established as a powerful analytical technique in compound identification and purity analysis in many fields including chemical synthesis and drug discovery. Modern LCMS instrumentation, along with walk-up software enables open access mass spectrometry (OAMS). OAMS refers to a MS that is available for use by investigators 24/7 without the intervention of MS facility personnel. It is easy-to-use and immediate accessible to chemists for reaction monitoring and it enables trainees to work at-will to analyze their samples in real-time. Walk-up service also gives trainees hand-on experience with state-of-the-art analytic machines and a greater appreciation for product characterization. More importantly, the self-service nature of the equipment is essential for our trainees to appreciate first-hand how to correlate chromatography fractions of synthetic and/or unknown compounds with MS analysis. In training students to characterize complex synthetic products, trainees in our department are driving innovation in terms of natural product synthesis, methodology development, catalysis of fine chemicals/advanced intermediates, and the characterization of new biological entities including polysaccharides and glycopeptides to name a few. This application supports a machine that serves the entire Chemistry Department of UBC. We train over 100 chemists working on organic, organometallic, inorganic, and materials chemistry. These trainees find jobs in industry and teaching and several PIs have received significant funding from industry while others are involved in company creation. This instrument, which will be supported by an in-kind contribution from the Chemistry department is critical to our training mission as it supports critically needed infrastructure to characterize compounds at all levels of the research enterprise.

总结。我代表几位同事，甚至整个不列颠哥伦比亚大学化学系，带头申请资金，为不列颠哥伦比亚省化学质谱学设施购买一台开放访问的液相色谱质谱计(OA-LCMS)，以更换一台已有17年历史的摇摇欲坠的仪器。质谱学(MS)是识别未知化合物和量化已知化合物的基本分析技术，因此对我们的培训任务至关重要。液相色谱-质谱仪(LC-MS)已成为化学合成、药物开发等领域中化合物鉴定和纯度分析的强有力的分析手段。现代的LC MS仪器和无人值守软件使开放式质谱学(OAM)成为可能。OAM是指无需MS设施人员干预即可供调查人员全天候使用的MS。它易于使用，化学家可以立即进行反应监测，并使受训者能够随意工作，实时分析他们的样品。无人值守服务还为学员提供了使用最先进的分析仪器的实践经验，并更好地欣赏了产品表征。更重要的是，设备的自助性对于我们的受训人员了解如何将合成和/或未知化合物的色谱组分与MS分析相关联是至关重要的。在培训学生对复杂的合成产品进行表征方面，我们系的实习生正在推动天然产品合成、方法开发、精细化学品/高级中间体的催化以及包括多糖和糖肽在内的新生物实体的表征等方面的创新。这个应用程序支持一台为整个UBC化学系服务的机器。我们培训了100多名从事有机、有机金属、无机和材料化学工作的化学家。这些学员在行业和教学领域找到了工作，一些私人投资机构从行业获得了大量资金，而其他人则参与了公司的创建。这一仪器将得到化学系的实物捐助的支持，这对我们的培训任务至关重要，因为它支持急需的基础设施，以在研究企业的各级表征化合物。