High resolution magic angle spinning NMR probe for biological tissue samples

用于生物组织样品的高分辨率魔角旋转 NMR 探头

• 批准号: RTI-2022-00536
• 负责人: Cahill, Lindsay
• 金额: $ 8.22万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=742971
• 关键词: resolution; magic; angle; spinning; NMR

Funding is requested for a high resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) probe. Compared to traditional NMR probes, the HR-MAS probe provides unparalleled signal sensitivity and spectral resolution. The HR-MAS probe, combined with the high field solid-state NMR spectrometer at Memorial University of Newfoundland, will become a powerful tool in the NMR facility and will benefit the research of many scientists across academic units. The unique technical design of the HR-MAS probe for solid tissue samples results in significantly narrow line shapes (similar to solution NMR experiments!) while maintaining the integrity of the biological tissue sample. The probe will enable funded research led by Dr. Lindsay S. Cahill (early career researcher in Chemistry) to study how chemicals in the brain and placenta change throughout gestation. This information is critical to improve our understanding of brain and placental function and allows identification of molecules that are essential for development of routine diagnostic tests during pregnancy. Dr. Cahill will accomplish these goals by studying brain and placental tissue samples from experimental mice and placental tissue samples from human pregnancies. This work has the potential to significantly enhance women's and fetal health through a better understanding of metabolism.

要求为高分辨率魔角旋转(HR-MAS)核磁共振(核磁共振)探测器提供资金。与传统的核磁共振探头相比，HR-MAS探头提供了无与伦比的信号灵敏度和光谱分辨率。HR-MAS探头与纽芬兰纪念大学的高场固态核磁共振光谱仪相结合，将成为核磁共振设施中的强大工具，并将有利于许多学术单位的科学家的研究。用于固体组织样品的HR-MAS探头的独特技术设计导致了非常窄的线形(类似于溶液核磁共振实验！)同时保持生物组织样本的完整性。这项调查将使由林赛·S·卡希尔博士(化学早期职业研究员)领导的资助研究能够研究大脑和胎盘中的化学物质在整个怀孕期间是如何变化的。这些信息对提高我们对大脑和胎盘功能的了解至关重要，并有助于识别孕期常规诊断测试所必需的分子。卡希尔博士将通过研究实验小鼠的大脑和胎盘组织样本以及人类妊娠的胎盘组织样本来实现这些目标。这项工作有可能通过更好地了解新陈代谢来显著提高妇女和胎儿的健康。