Control of mRNA stability and translation by G3BP RNA-binding proteins

G3BP RNA 结合蛋白控制 mRNA 稳定性和翻译

• 批准号: RGPIN-2018-06485
• 负责人: Smibert, Craig
• 金额: $ 5.25万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744145
• 关键词: Control; mRNA; stability; translation; G3BP

The growth and development of all multi-celled organismsincluding humansrequires that cells within the body adopt specialized functions. Cell specialization involves the presence of different proteins in different cell types (e.g. muscle proteins in muscle cells, liver proteins in liver cells, etc.). Cells produce individual proteins using specific signals known as mRNAs in a process called translation. We are studying two strategies that are employed to control which proteins are present in any particular cell. In one strategy, translation of specific proteins within a cell can be turned on or off. In the other strategy, the production of a protein can be blocked by destroying the corresponding mRNA via mRNA degradation. The decision to translate or degrade an mRNA is controlled by proteinscalled RNA-binding proteins (RBPs)that bind mRNAs. The work in this proposal is aimed at understanding how RBPs stabilize mRNAs and turn on their translation. These types of mechanisms function throughout the life span of all multi-celled organisms and play particularly prominent roles during the earliest stages of development. We are using a simple organismthe fruit flyto study how RBPs are controlled during the earliest stages of the organism's life. Since there are many similarities between how flies and humans grow and develop, our work is applicable to humans.

包括人类在内的所有多细胞生物的生长和发育都要求体内的细胞具有特殊的功能。细胞特化涉及不同细胞类型中不同蛋白质的存在（例如，肌肉细胞中的肌肉蛋白，肝细胞中的肝蛋白等）。细胞在翻译过程中使用被称为mrna的特定信号产生单个蛋白质。我们正在研究两种策略，用来控制任何特定细胞中存在的蛋白质。在一种策略中，细胞内特定蛋白质的翻译可以打开或关闭。在另一种策略中，可以通过mRNA降解破坏相应的mRNA来阻止蛋白质的产生。翻译或降解mRNA的决定是由一种叫做rna结合蛋白（rbp）的蛋白质控制的，它与mRNA结合。本提案的工作旨在了解rbp如何稳定mrna并开启其翻译。这些类型的机制在所有多细胞生物的整个生命周期中发挥作用，在发育的最初阶段起着特别突出的作用。我们正在使用一种简单的生物——果蝇——来研究rbp在该生物生命的最初阶段是如何被控制的。由于苍蝇和人类的生长发育有许多相似之处，我们的工作也适用于人类。