CoastCheck: Establishment of robust eDNA assays to assess organic pollution impacts on infaunal macrobenthic invertebrate communities in coastal waters of British Columbia

省级和联邦政府授权的废水和环境影响监测项目要求对生活在海底软沉积物中的海洋无脊椎动物群落的健康状况进行评估。bi利用传统的分类和视觉分类方法为政府和行业提供了20多年的分类鉴定服务。然而，这种方法非常耗时、昂贵，并且在技术上具有挑战性。有必要扩大和提高监测这些无脊椎动物的可靠性。所有生物体都是由它们的遗传物质决定的，这些DNA被释放到环境中。这种“环境DNA （eDNA）”可以通过对水和沉积物样本进行敏感和准确的分子测试来检测。该项目的总体目标是开发可靠的、有针对性的基于edna的测试，以评估有机污染对生活在海洋地区沉积物中的无脊椎动物的影响。CRD项目结合了学术（维多利亚大学）、工业（生物）和废水管理服务提供商（维多利亚首都地区和大温哥华污水和排水区）的专业知识，以实现两个主要目标：1)开发和验证对感兴趣的无脊椎动物物种的验证标本的eDNA测试；2)创建一个强大的，标准化的eDNA现场采样方法，用于使用生活在海底沉积物中的无脊椎动物来评估海洋沿海社区的有机污染影响。这将通过三个具体目标来实现：1)从经过验证的标本中获得高质量的DNA序列，2)开发和验证选定无脊椎动物的eDNA测试，以及3)将eDNA测试应用于现实场景。这些新的eDNA工具的常规应用将彻底改变分类服务行业和废水环境影响的评估方式。

Provincially- and federally-mandated programs for wastewater and environmental effects monitoring require an assessment of the health of marine invertebrate communities that live in the soft sediments at the sea bottom. Biologica has provided taxonomic identification services for government and industry for over 20 years using traditional sorting and visual classification methods. However, this approach is very time-consuming, costly, and technically challenging. There is a need to expand and improve upon the reliability of monitoring these invertebrates. All living organisms are defined by their genetic material and this DNA is released into the environment. This "environmental DNA (eDNA)" can be detected using sensitive and accurate molecular tests on water and sediment samples. The overall project goal is to develop robust, targeted eDNA-based tests to assess the impact of organic pollution on invertebrates that live in sediments of marine areas. The CRD project combines the expertise of academic (University of Victoria), industry (Biologica), and wastewater management service providers (the Capital Regional District of Victoria and the Greater Vancouver Sewerage and Drainage District) to address two main objectives: 1) to develop and validate eDNA tests for verified specimens of invertebrate species of interest, and 2) to create a robust, standardized eDNA field sampling approach for marine coastal community assessments of organic pollution impacts using invertebrates that live in sea bottom sediments. This will be accomplished in three specific aims: 1) to obtain high quality DNA sequences from verified specimens, 2) to develop and validate eDNA tests for select invertebrates, and 3) to apply the eDNA tests in real-world scenarios. The routine application of these new eDNA tools will revolutionize the taxonomy service industry and how environmental impacts of wastewater are assessed.