Cellular Interactions in Mammary Gland Development

乳腺发育中的细胞相互作用

• 批准号: RGPIN-2020-05726
• 负责人: Plante, Isabelle
• 金额: $ 3.42万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=757480
• 关键词: Cellular; Interactions; Mammary; Gland; Development

The development of the mammary gland is a multistage process tightly orchestrated by hormones, and mainly occurring during puberty, pregnancy and lactation. It also requires a constant and controlled bidirectional crosstalk between the cells that form the gland. We have shown that the composition of the junctional nexus involved in these interactions varies in a stage-dependent manner, thus suggesting stage-dependent role and regulation of the junctions' components. However, while many of those components have been identified, many others remain to be discovered. More importantly, the mechanisms controlling the arrangement and function of the junctional nexus throughout mammary gland development are poorly understood. The main objective of this research program is thus to identify those mechanisms, focusing on hormones and interacting proteins. In addition, some environmental pollutants are similar to hormones and can mimic their actions. These pollutants are called endocrine disruptors. Understanding the mechanisms by which an exposure to endocrine disruptors can modulate the establishment and function of cellular interactions will allow us to determine how critical those mechanisms are for the normal development and function of the mammary gland. To achieve our research objectives, we will use innovative models to mimic the mammary gland structure developed by our lab combined with animal models. Together, results from this research program will lead to a better understanding of mammary gland physiology, function and organogenesis, particularly of how hormones and cellular junctions are governing the development of the mammary gland. We will also improve the scientific knowledge related to cellular interactions and crosstalk.

乳腺的发育是一个由激素紧密调控的多阶段过程，主要发生在青春期、妊娠期和哺乳期。它还需要形成腺体的细胞之间有一个恒定的、可控的双向串扰。我们已经证明，参与这些相互作用的连接连接的组成以阶段依赖的方式变化，从而表明连接成分的作用和调节是阶段依赖的。然而，虽然其中许多成分已被确定，但仍有许多成分有待发现。更重要的是，在整个乳腺发育过程中，控制连接连接的排列和功能的机制尚不清楚。因此，这项研究计划的主要目标是确定这些机制，重点是激素和相互作用的蛋白质。此外，一些环境污染物与激素相似，可以模仿它们的作用。这些污染物被称为内分泌干扰物。了解暴露于内分泌干扰物可以调节细胞相互作用的建立和功能的机制，将使我们能够确定这些机制对乳腺的正常发育和功能有多重要。为了实现我们的研究目标，我们将使用创新的模型来模拟我们实验室开发的乳腺结构，并结合动物模型。总之，该研究项目的结果将有助于更好地理解乳腺生理学、功能和器官发生，特别是激素和细胞连接如何控制乳腺的发育。我们还将提高与细胞相互作用和串扰相关的科学知识。