Cryopreservation and polyploid breeding strategies for Cannabis sativa

大麻的冷冻保存和多倍体育种策略

• 批准号: 576989-2022
• 负责人: Jones, AndrewMP
• 金额: $ 10.56万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=763532
• 关键词: Cryopreservation; polyploid; breeding; strategies; Cannabis

Cannabis has become one of the most economically important crops in Canada and it contributed billions of dollars to the GDP each year. Despite this, the long history of prohibition has resulted in a general lack of modern research and in comparison to other crops, there is a deficit in our basic understanding of the plants biology and biotechnological tools. This is particularly true in the case of breeding technologies, where there is great need for genetic preservation systems and potential for the development of seedless triploids or higher ploidy levels. Preliminary reports are promising, but there remains many questions on the implications and potential of this approach. In this project, we aim to address some of these deficiencies by refining cryopreservation methods to facilitate the long term conservation of clonal lines and evaluate polyploid breeding methodologies for genetic improvement in cannabis. These projects will be coupled with advanced metabolomic and genomic tools to gain in depth understandings of the genetic fidelity of cryopreserved material, and detailed information on the impacts of increased ploidy. These experiments will provide new insights into cannabis biology and ensure that the Canadian Cannabis industry remains globally competitive as the industry expands.

大麻已成为加拿大最重要的经济作物之一，每年为国内生产总值贡献数十亿美元。 尽管如此，长期的禁令导致普遍缺乏现代研究，与其他作物相比，我们对植物生物学和生物技术工具的基本理解存在不足。 在育种技术方面尤其如此，因为在这方面非常需要遗传保存系统，并有潜力开发无核三倍体或更高的倍性水平。 初步报告是有希望的，但对这一办法的影响和潜力仍有许多疑问。 在这个项目中，我们的目标是通过改进冷冻保存方法来解决其中的一些缺陷，以促进克隆系的长期保存，并评估用于大麻遗传改良的多倍体育种方法。 这些项目将与先进的代谢组学和基因组学工具相结合，以深入了解冷冻保存材料的遗传保真度，并详细了解倍性增加的影响。 这些实验将为大麻生物学提供新的见解，并确保加拿大大麻行业在行业扩张时保持全球竞争力。