基于periostin-TGF-β环路对成纤维细胞增殖、迁移及分泌功能的调控探讨散结祛瘢方治疗瘢痕疙瘩的作用机制

瘢痕疙瘩（Keloid）是一种具有侵袭性生长特点的良性皮肤肿瘤，其临床发病率高，不仅影响美观，还引发功能障碍，甚至癌变。瘢痕疙瘩的治疗方法主要有手术、放疗、注射等，但效果有限，易复发。瘢痕疙瘩成纤维细胞（KFB）是瘢痕疙瘩发病的关键细胞。骨膜蛋白（periostin）可诱导KFB增殖、迁移及分泌信号的转导，促进KFB分泌TGF-β1，TGF-β1又促进KFB分泌periostin，形成periostin-TGF-β“恶性循环”环路。中医认为气滞血瘀、瘀而生毒是瘢痕疙瘩的核心病机，治以活血化瘀、软坚散结。我院中药协方散结祛瘢方治疗瘢痕疙瘩临床疗效显著，并能降低瘢痕疙瘩裸鼠模型瘢痕组织中TGF-β1的表达。故本项目通过临床、动物、体外层面，从调控periostin-TGF-β环路抑制KFB增殖、迁移及分泌，探讨散结祛瘢方治疗瘢痕疙瘩的效应机制，为瘢痕疙瘩防治提供新的思路和方法。

Keloid is a benign skin tumor with aggressive growth characteristics. Its clinical incidence is high, which not only affects the appearance, but also causes skin dysfunction and even cancer. The main treatment methods of keloid are surgery, radiotherapy, injection, etc., but the effect is limited and easy to recur. Keloid fibroblasts (KFB) are key cells in the pathogenesis of keloids. Periostin can induce KFB proliferation, migration and secretion signal transduction, and promote KFB to secrete TGF-β1, which in turn promotes KFB to secrete periostin, forming a periostin-TGF-β "vicious circle" loop. Traditional Chinese medicine believes that qi stagnation and blood stasis, stasis and toxins are the core pathogenesis of keloids. Sanjie Quban Formula has a significant clinical effect on keloids, and can reduce the expression of TGF-β1 in nude mice model of keloids. Therefore, this project aims at regulating the periostin-TGF-β loop and inhibiting the proliferation, migration and secretion of KFB through clinical, animal and in vitro levels, and explores the effect mechanism of Sanjie Quban Formula in the treatment of keloids, so as to provide new ideas and methods for the prevention and treatment of keloids.