人工回灌过程中含水介质微生物-悬浮物复合堵塞的形成机制

人工回灌是解决地下水超采问题的有效措施，但人工回灌过程中含水介质的堵塞问题，是限制回灌技术应用最主要的技术瓶颈。本项目以人工回灌过程中含水介质微生物-悬浮物复合堵塞的形成机制为研究目标，通过静态批量试验和动态渗流砂柱试验，模拟人工回灌含水介质的过程。利用原子力显微镜、激光共聚焦扫描电镜、X射线断层扫描和微生物分子生物学等手段，分析微生物在含水介质表面的成膜过程及其对含水介质性质的影响；弄清含水介质表面微生物和胞外聚合物与回灌水中悬浮颗粒物的交互作用过程，探明微生物和胞外聚合物对人工回灌过程中悬浮颗粒物运移、沉积和含水介质渗透性的影响；厘清回灌水性质、回灌方式、水动力条件对微生物-悬浮物复合堵塞体形成过程、稳定性和脱落难易程度的影响，明晰回灌各影响因素-微生物生长代谢特征参数-悬浮颗粒物沉积之间的响应关系，以期能够为解决人工回灌过程的复合堵塞问题提供理论依据。

Artificial groundwater recharge is an effective method to solve the groundwater overexploitation. However, the clogging of aquifer during recharge limits the application of artificial recharge. In this study, we aim to clarify the mechanisms of complex clogging in porous media under the coexistence of microbes and suspended particles. Through a combination of batch/column experiments and multiple technologies (such as atomic force microscopy, confocal laser scanning microscope, X-ray micro-computed tomography facility and high-throughput sequencing), we aim to explore the formation process of biofilm on the porous media and its effect on the characteristics of porous media. The interaction between different kinds of microbes (or extracellular polymeric substances) and suspended particles will be explored, and the impact of these interactions on the transport and retention of suspended particles and the hydraulic conductivity of porous media will be clarified. The effects of recharge water, recharge methods, and hydrodynamic conditions on the formation, stability, and detach of microbes-particle combined substances will be investigated. The response relationship among influencing factors of recharge, parameters of microbial characteristic, and deposition of suspended particles will be identified. This study aims to provide theoretical foundation for the solution of complex clogging during artificial recharge.