NSCLC外泌体lncRNATERC的临床价值及其m6A甲基化修饰调控NETs形成的机制

非小细胞肺癌（NSCLC）缺乏精准有效的肿瘤标志物。外泌体（exosome）携带的长链非编码RNA（lncRNA）是极具前景的NSCLC精准诊断标志物。我们前期研究发现，NSCLC细胞分泌的exosome中lncRNA TERC（lncTERC）显著富集，在NSCLC患者血浆中呈高表达；lncTERC上存在m6A甲基化修饰，并可通过exosome进入中性粒细胞，影响其信号网络和自噬，与中性粒细胞胞外陷阱（NETs）形成密切相关。据此推测：exosomelncTERC是NSCLC诊断、疗效监测和预后判断的新型液体活检标志物；m6A甲基化修饰介导lncTERC参与NETs形成，促进NSCLC生长。拟通过临床标本及体内外实验研究exosome lncTERC在NSCLC中的临床价值，阐明lncTERC参与调控中性粒细胞功能的具体机制，为揭示NSCLC发病机理及精准诊疗提供新的思路和策略。

Non-small cell lung cancer (NSCLC) lacks precise and effective tumor markers. Long non-coding RNA (lncRNA) carried by exosome is a promising diagnostic marker for NSCLC. Our previous study found that lncRNA TERC (lncTERC) was significantly enriched in exosome secreted by NSCLC cells and was highly expressed in the plasma of NSCLC patients. There is m6A methylation modification on lncTERC, which can enter neutrophils through exosome, affect their signal network and autophagy, and is closely related to the formation of neutrophils extracellular traps (NETs). Therefore it is hypothesized that exosomal lncTERC is a novel liquid biopsy marker for diagnosis, efficacy monitoring and prognosis of NSCLC. M6A methylation mediated lncTERC to participate in the formation of NETs and promote the growth of NSCLC. It is intended to study the clinical value of exosomal lncTERC in NSCLC through clinical specimens and in vivo and in vitro experiments, to elucidate the specific mechanism of lncTERC participating in the regulation of neutrophil function, and to provide new ideas and strategies for revealing the pathogenesis and precise diagnosis and treatment of NSCLC.