毛红椿克隆繁殖事件对种群多样性影响及分子驱动机制研究

克隆植物种群遗传结构和多样性受到克隆繁殖方式影响，根萌克隆繁殖中SPL基因受到miR156靶向调控影响植物激素合成代谢从而控制根萌芽的形成。毛红椿作为能够克隆繁殖的濒危二级保护树种，通过根萌的方式进行克隆生长。毛红椿克隆繁殖对种群影响以及现象背后的关键驱动基因还不清楚。前期调研发现不同种群根萌发生率不同，猜测毛红椿克隆繁殖对种群结构和多样性有一定影响，且克隆繁殖发生可能受到SPL/miR156类似的分子调控机制驱动。为证实以上假说，九连山、官山自然保护区的毛红椿作为研究对象，通过分子标记分析不同种群结构和遗传多样性，利用高通量测序筛选关键基因，构建超表达载体并转基因验证候选基因功能。为毛红椿植被恢复与保持等研究提供科学的依据以及拓展毛红椿克隆繁殖育种的手段。

The genetic structure and diversity of clonal plant population are affected by its clonal propagation. In root sprouting clonal plant, SPL gene is regulated by microRNA156, which affects phytohormone anabolism controlling the formation of root sprout. Toona ciliata, an endangered secondary protection species and clonal plant, can be cloned by root sprout. The effect of clonal propagation on the population and the key genes driving the phenomenon are still unclear in this species. Our previous work found that the incidence of root sprouting was different among different populations. It is therefore speculated that clonal propagation of T. ciliata has a certain impact on population structure and diversity, and clonal propagation may be driven by similar molecular regulation mechanism of SPL/miR156. To confirm these, T. ciliata growing in Jiulianshan and Guanshan Nature Reserves were selected as the research materials. Molecular markers will be used to analyze the population structure and genetic diversity of different populations. Addtionally, the high throughput sequencing will be appled to generate the key genes. Then, we will construct overexpression vectors and transfer genes to verify the function of candidate genes. Of these, it may provide a scientific basis for the research of vegetation restoration and conservation, and expand the means of clone breeding of T. ciliata.