甜瓜细胞核雄性不育具有育性稳定、配置组合自由的特点，对于育种工作具有重要的研究意义。本研究利用甜瓜细胞核雄性不育ms5 为材料，在前期对雄性不育基因精细定位的基础上，确定了花粉发育特异表达基因AMS(ABORTED MICROSPORES)为甜瓜细胞核雄性不育候选基因，该基因编码bHLH类转录因子，且该基因缺失后会导致花粉不能正常形成，最终导致雄性不育的发生。研究采用酵母单杂技术、染色质免疫共沉淀测序技术挖掘AMS 转录因子调控的关键靶基因，结合对雄性不育植株与可育株不同发育时期雄蕊的转录组测序分析，明确AMS 转录因子调控的关键基因及其参与的代谢途径；利用基因沉默技术及AMS 转化拟南芥ams 突变体鉴定基因功能，在DNA、RNA和蛋白质等水平上，系统的研究分析AMS在花药发育的过程中的主要作用、绘制AMS 转录因子调控花药发育的网络系统、明确甜瓜雄性不育的分子机理。

Single Nuclear genetic male sterility has stable male sterility and crossing freedom characterize, it is an important germplasm for breeding program. Previous research on fine mapping of male sterility gene in melon identified Aborted Microspores (AMS) expressed gene is the candidate gene for melon male sterility. This gene coding bHLH transcription factor, missing this gene will induce abnormal pollen and male sterility. In the present research, using Yeast one-hybrid technology, Chromatin immunoprecipitation- sequencing investigate the target genes which regulated by AMS transcription factor, combination with transcription sequence on different development stages of stamen on male sterility plants and male fertility plants, identify biological pathway which AMS transcription factor and its regulated target genes involved. Gene silencing and Arbidopsis trans-gene methods will be used for identify gene function. These research will help us to know how the AMS transcription factor regulate the stamen develop in DNA, RNA and protein level, net of AMS regulate the stamen develop, molecular mechanism of male sterility in melon will be identified.