脂基化修饰是一类广泛存在的蛋白翻译后修饰。由于脂质分子独特的物理化学性质赋予了蛋白质特殊的结构和功能，因此开展蛋白质翻译后脂基化修饰结构与功能分析对于细胞交叉调控信号转导通路等机制研究具有重要的意义。研究表明哺乳动物体内脂基化修饰的来源多数是内源性活化的脂肪酰辅酶A（Acyl-CoA）。但是目前针对脂肪酰CoA这类重要的调控脂基化反应的辅助因子，其相应的检测技术极度匮乏。因此本项目的研究关键和重心是针对长链脂肪酰CoA发展快速、空间特异性，实时动态的荧光蛋白探针技术。随后将该生物荧光探针应用于多种肿瘤细胞和正常细胞内长链脂肪酰CoA的监测，希望可以鉴定出长链脂肪酰CoA含量存在的细微差别，同时实现在单细胞和活体动物水平对长链脂肪酰CoA的高时空分辨检测和成像。该探针的发现也将为更好地研究肿瘤内的长链脂基化修饰蛋白的调控机制，提供重要的创新工具。

Fatty acylation modifications are a broad class of post-translational modifications of proteins. Since the unique physical and chemical properties of lipids conferring specific structures and functions to target proteins, it is important significance to study the structure and function analysis of protein fatty acylation for multi-level cross signal transduction pathways. Researches have demonstrated that most of the sources of acylation modification are endogenously activated fatty acyl-CoA in mammals. However, the corresponding detection techniques for the important cofactors of acyl reaction such as fatty acyl-CoA are extremely scarce. Therefore, the key and focus of this project is to develop rapid, spatially specific, real-time, dynamic biosensor for fatty acyl-CoA detection. Meanwhile，this biosensor is applied to the monitoring of long-chain fatty acyl-CoA in various tumor cells and normal cells, and it is hoped that the nuances of the long-chain fatty acyl-CoA concentration can be identified, and it can be achieved high temporal and spatial resolution imaging of long chain fatty acyl-CoA in single cell and animals. The discovery of this new probe will also provide an innovative tool for studying the regulation mechanism of long-chain lipid-modified proteins in tumor.