γ-氨基丁酸在肝移植术后急性排斥反应中的作用及机制研究

γ-氨基丁酸（GABA）是中枢神经系统最重要的一种抑制性神经递质，近来发现其具有免疫抑制功能，可以抑制T细胞的活化，并可诱导调节性T细胞的产生，我们前期研究显示，GABA可抑制已活化CD8+T细胞的增殖，而CD8+T细胞是肝移植术后急性排斥反应中最重要的效应细胞，因此我们推测GABA很有可能在器官移植术后急性排斥反应中发挥重要作用。本研究拟通过建立大鼠肝脏移植急性排斥反应模型，经GABA灌胃给药，评估GABA对大鼠肝移植术后急性排斥反应的免疫抑制疗效，设计体内、体外等一系列实验，经HE染色、流式细胞术、CBA、ELISA、RT-PCR、westernblot等方法来深入阐释GABA在肝移植术后急性排斥反应中的疗效以及可能机制。从而进一步探讨GABA成为新型免疫抑制剂的可能性。

γ-Aminobutyric acid (GABA) is a key inhibitory nerve transmitter. It was reported that GABA had an property of immnunosuppression. It can inhibit the activation of T cells and induced the production of T regulatory cells. In our previous study, we found that γ-aminobutyric acid (GABA) can inhibit the proliferation of activated CD8+T cells. It was known that CD8+T cells were main effective cells in transplantation rejction. So we hypothesized, GABA may play an important role in process of rejection after organ transplantation. In the present study, we try to investigate the role of GABA in acute rejection after liver transplantation from animal model, in vitro and in vivo by Hematoxylin-eosin staining, Flow cytometry,Cytometric Bead Array,Enzyme-linked immunosorbent assay,Reverse-transcription Polymerase Chain Reaction and Westernblot. With the implementation of the project, it is of great importance to clarify the possibility of GABA as an new immunosuppressant in organ transplantion.