番茄是新疆一种重要的特色经济作物，在新疆种植番茄具有得天独厚的优势。然而随着世界贸易的日益深入，番茄面临日趋严重的各种植物双生病毒的危害，而目前已有的技术方法难以在生产中实现番茄对多种双生病毒的广谱抗性。因此培育广谱抗双生病毒番茄品种具有重要意义。CRISPR/Cas9系统是细菌抵抗噬菌体侵染的一种免疫系统。本项目将首次把CRISPR/Cas9系统引入植物用于抗病毒育种。充分利用CRISPR/Cas9系统的特点，以不同双生病毒基因组序列中存在的高度保守区段作为Cas9的靶位点，构建CRISPR/Cas9系统，导入番茄中，以期实现番茄广谱抗双生病毒育种的目标，为新疆番茄高产稳产奠定重要基础。

Tomato is a kind of important characteristic economy crops in xinjiang. It is very suitable for the growth of tomato in xinjiang. However, with the increasingly deepening of world trade, tomatoes are facing increasingly severe the dangers of all kinds of plant geminivirus. However, the existing technology and methods is difficult to realize broad-spectrum resistance to geminivirus in tomato. Therefore it has important practical significance to develop broad-spectrum geminivirus-resistant tomato. CRISPR/Cas9 system is one of immune systems which were used to defend bacteriophage in bacteria. It is first that CRISPR/Cas9 system will be used for antiviral breeding of tomato in this project. There are several highly conserved regions in different geminivirus genomes. In order to achieve broad-spectrum anti-geminivirus breeding of tomato, these conserved regions are used as the target of Cas9 in CRISPR/Cas9 system, which will establish important basis for high and stable yield of tomato in xinjiang.