小麦条锈菌为专性寄生菌，选用抗病品种是防治条锈病最为有效措施，然而小麦抗条锈病的机制还有许多方面尚未搞清。我们在前期工作中，发现微丝骨架受微丝结合蛋白的调控,微丝骨架的解聚导致小麦抗病性降低；并克隆了促进微丝解聚的小麦微丝结合蛋白基因TaPRO和TaADF1，它们在非亲和互作中表达下调。本项目将以TaPRO等促进微丝解聚的微丝结合蛋白基因为切入点，通过病毒诱导的基因沉默技术揭示其在小麦抗条锈病中的作用；利用qRT-PCR进行表达谱分析；Western blot检测在蛋白水平上的表达差异；通过亚细胞定位和免疫细胞化学定位，确定其作用位点以及时空变化特征；并转化拟南芥研究这些基因的功能。本研究有望从全新的角度阐明小麦抗条锈性机制，为该病防治和小麦抗锈育种提供新思路。

Puccinia striiformis f. sp. tritici is an obligate fungus. Cultivating resistant cultivars is the most effective measure to prevent rust disease in wheat. However, many aspects of the resistance mechanism of wheat against P. striiformis f. sp. tritici remain to be elucidated. In previous studies, we found that actin filaments are regulated by actin-binding proteins (ABPs) and the depolymerization of actin filaments resulted in a weakening of wheat resistance. The full-length of the ABP genes TaPRO and TaADF which promote the depolymerization of actin filaments have been successfully cloned. The expression of these genes is downregulated in incompatible interactions. In this project, we attempt to reveal the roles of TaPRO and other ABPs which promote the depolymerization of actin filaments in the resistance of wheat against P. striiformis f. sp. tritici by means of virus-induced gene silencing; and use qRT-PCR to analyze the expression profile; the difference in the expression of the genes will be detected by Western blot; their functional loci and spatiotemporal characteristics are elucidated by means of subcellular localization and immunocytochemical localization; the functions of these genes will be investigated in transgenetic Arabidopsis plants. This research is aimed at clarifying the resistant mechanism of wheat against the stripe rust from a new angle, generating novel ideas of controlling wheat stripe rust and wheat resistance breeding.