茵陈蒿汤作为治疗黄疸的经典方，临床疗效良好，但其利胆退黄作用机制和药效物质基础目前尚不明确。本项目基于茵陈蒿汤和胆红素体内代谢规律的相关性，推测茵陈蒿汤有效成分体内的葡萄糖醛酸化过程可调节“黄疸”大鼠体内胆红素代谢关键酶和转运子的活性，促进胆红素代谢。研究内容为：采用HPLC-Q/TOF技术建立茵陈蒿汤在正常和黄疸大鼠体内的代谢物谱；采用现代分析与生物酶解技术，以茵陈蒿汤为模型药物，建立以Ⅱ相结合反应为主要代谢途径的利胆退黄中药的药代动力学研究方法，并用于茵陈蒿汤在正常与黄疸大鼠体内药代动力学差异研究，阐明其体内药代动力学规律。最后，通过研究茵陈蒿汤、有效成分及其主要代谢物对胆红素代谢关键酶（UGT1A1和β-GCD）和转运子（Mrp2和Bsep）的调节作用，结合药效作用部位的效应成分含量与利胆作用的相关性，探索其体内药效物质基础及作用机制，为其他利湿退黄中药的体内过程研究提供思路和方法。

As a classic prescription in the treatment of jaundice, Yinchenhao decoction has a satisfactory clinical effect. But the mechanism of removing jaundice and the pharmacodynamic substance foundation of Yinchenhao decoction is not clear, presently. In this project, we speculate the choleretic effect of Yinchenhao decoction would be related to the glucuronidation process of its active ingredients in rats based on the correlation of Yinchenhao decoction and bilirubin metabolic processes. And the mechanism is maybe related to adjustment of key bilirubin metabolic enzymes and transporters. This hypothsesis will be confirmed by the following researches. At first, the in-vivo metabolic spectrum of Yinchenhao decoction in normal rats and jaundice rats will be studied by HPLC-Q/TOF technology. Then taking Yinchenhao decoction as a model drug, using both HPLC-MS/MS and enzymolysis biology technologies to establish a method for pharmacokinetics of chinese medicines which mainly remove jaundice and are metabolized in phase Ⅱ. In addition, it also will be applied to analyze the pharmacokinetics of Yinchenhao decoction in normal rats and jaundice rats. At last, the induction effects of Yinchenhao decoction, its active ingredient and its major metabolites on the key bilirubin metabolic enzymes (UGT1A1 and β-GCD) and transporters (Mrp2 and Bsep) will be studied. And the relationship of major active ingredients and metabolites’ concentration at site of action with its choleretic effect will also be studied to explore the mechanism and pharmacodynamic substance foundation of Yinchenhao decoction in vivo. This study will lay the foundation for further researches of other different Chinese medicines which have the same effects of curing jaundice.