基于背根神经节 P2X3受体调控的电针干预糖尿病神经痛作用研究

糖尿病神经痛(DNP)是糖尿病最常见的并发症之一，主要表现为自发性烧灼样疼痛、痛觉敏化，严重影响患者生存质量。目前药物治疗效果不佳。针灸(包括电针)已逐步用于治疗DNP，初步发现电针治疗DNP存在低频作用好于高频的现象。研究表明背根神经节(DRG) P2X3受体在DNP中起着重要作用，并受PKC调制。本项目拟通过高低频率电针效应对比，进一步明确低频电针干预DNP的优势作用；在此基础上，检测DRG神经元P2X3受体介导的快反应电流强度，和P2X3受体总蛋白和细胞膜表达水平，观察低频电针对DRG P2X3受体功能、表达和从胞质向胞膜转移的影响，结合低频电针对DRG PKC的调节，以及P2X3受体、PKC激动剂对低频电针作用的影响，阐明低频电针干预DNP的DRG P2X3受体调控机制及其通过PKC的干预途径，为电针治疗DNP及其频率选择提供科学依据和阐释，以期推动电针更广泛、更好地治疗DNP。

Diabetic neuropathic pain (DNP) is one of the most common complications of diabetes. It is characterized by spontaneous burning pain and hyperalgesia, and severely affects the patients' life quality. Currently, there is no satisfied drug treatment. Acupuncture including electroacupuncture (EA) is now gradually being applied to treat DNP, with a phenomenon that the effect of EA with low frequency is better than that of EA with high frequency. Studies show that dorsal root ganglion (DRG) P2X3 receptor plays an important role in DNP, which is regulated by PKC. The project is to further determine the superiority of EA with low frequency in treating DNP by comparing the effects of EA with high frequency and low frequency. P2X3-mediated fast-inactivating current of DRG neuron, DRG P2X3 total protein level and its expression in cell membrane are measured to evaluate the effects of EA with low frequency on DRG P2X3 function, expression and its shift from cytoplasm to cell membrane. In addition, the regulation of EA with low frequency of DRG PKC, and the effects of P2X3 and PKC agonists on the action of EA with low frequency are also studied, so as to explore the mechanism of DRG P2X3 regulation through PKC pathway by EA with low frequency in the treatment of DNP, which will provide scientific evidence and explanation for DNP treatment by EA and its frequency selection, to promote EA more widely and more effectively treating DNP.