基于代谢组学和蛋白组学的朝医方“千金文武汤”治疗糖尿病肾病机制研究

糖尿病肾病 (DKD)是糖尿病引起的严重和危害性最大的一种慢性并发症，是胰岛素依赖型糖尿病患者主要死因。目前尚无特效治法和直接针对DKD的有效药物。朝医传统方剂“千金文武汤”（葛根、山药、黄芩、藁本、麦门冬、五味子、桔梗、升麻、白芷）治疗DKD临床疗效显著，并在安全性、有效性、可靠性、有效成分等方面进行了研究，但目前还缺乏现代药物学研究，尚未阐明该方剂多靶点作用特点和作用机制。本课题是在前期基础上进行的进一步探索研究，利用代谢组学和蛋白组学等系统生物学方法对“千金文武汤”药物作用的分子机制进行实验研究，解析其治疗DKD的作用机制及其作用靶点，初步阐明其调节及修复机制；同时评价在研究朝药方剂的分子生物学机制方面，传统方法和现代系统生物学联合应用的优越性。通过对复方民族药物治疗机制研究进行探索和创新，有助于加快现代医学的先进技术在民族药研究中的应用。

Diabetic kidney disease (DKD) is a serious and dangerous complication caused by diabetes mellitus and also is the major cause of death in the insulin dependent diabetes mellitus (IDDM) patients. There is no specific treatment and effective medicine for DKD. Traditional Korean medicine prescription "Qianjin Wenwu Decoction" (puerarin, yam, Radix Scutellariae and Rhizoma ligustici, ophiopogon root, Schisandra, Balloonflower root, Cimicifuga rhizome, dahurian angelica root) has a therapeutic effect in the treatment of DKD. Its safety, validity, reliability, and active ingredients have been investigated. But there is a lack of modern pharmacological research to elucidate the multi-targeting features and mechanism of action for this prescription. Based on our previous studies, this experimental study will investigate the molecular mechanism of "Qianjin Wenwu Decoction" in the treatment of DKD and explore the mechanisms and targets of treatment and clarify the regulation and repair mechanism by using metabolomics and proteomics methods; at the same time, this study will also evaluate the superiority of combination of traditional methods and modern system biology methods in the research of the molecular biology mechanism of traditional Korean medicine prescription. The exploration and innovation in the study of the therapeutic mechanism of compound ethnic medicine will accelerate the application of advanced technology of modern medicine in the study of ethnic medicine.