“舒心饮”经内质网应激及其介导的氮氧自由基平衡改善冠脉内皮功能紊乱的研究

冠心病严重危害着人类健康，对其防治成为了心血管疾病临床和基础研究的重点。冠状动脉粥样硬化是冠心病的主要病理机制，内皮功能紊乱是其发生的始动环节，并伴随着疾病发展的全过程。本课题组前期研究表明，“舒心饮”防治冠心病临床疗效确切，有效保护血管内膜，但其分子作用机制尚不完全清楚。本研究以内皮功能紊乱为切入点，在血管内皮细胞特异性标记绿色荧光蛋白转基因斑马鱼模型和大鼠冠脉血管环模型上，评价“舒心饮”改善内皮功能紊乱的作用；在冠脉内皮细胞模型上，围绕内质网应激及其介导的氮自由基NO和氧自由基ROS平衡，探讨“舒心饮”改善内皮功能紊乱的分子作用机制；基于细胞模型上分子作用机制，在斑马鱼和冠脉血管环模型上，用特异性的抑制剂或者激动剂从反面验证“舒心饮”改善内皮功能紊乱的机制。该研究为临床上“舒心饮”防治冠心病提供了基础研究支持，对冠心病的中医药防治和“舒心饮”的进一步开发具有重要意义。

The prevention and treatment of coronary heart disease, a serious disease, has become an important field of both clinical and basic research. Coronary atherosclerosis occurs in the early stage of coronary heart disease. Endothelial dysfunction initiates coronary atherosclerosis and exists in the entire process of disease development. Our previous studies indicated that the efficiency of Shuxinyin for coronary heart disease and vascular endothelial cell damage while the molecular action mechanism is not fully elucidated. This project is focused on the protective effect of Shuxinyin on endothelial dysfunction on rat coronary arteries and zebrafish which specific expresses GFP in endothelial cells. Furthermore, the molecular action mechanism of Shuxinyin suppressing the endothelial dysfunction will be found on both rat primary coronary endothelial cells and coronary arteries. This study proposes that the molecular mechanism of Shuxinyin protect endothelial dysfunction via regulating endoplasmic reticulum stress-mediated nitrogen and oxygen free radicals balance. This research can provide research support and evidence for clinic usage of Shuxinyin for prevention of coronary heart disease. Thus, this study is important for the further development and usage of traditional Chinese medicine for coronary heart disease treatment.