血液中蛋白的唾液酸糖基化状态与人体的癌症疾病密切相关，开展唾液酸糖基化研究将有利于疾病的早期诊断。然而常规糖基化研究是先将所有类型的糖肽进行富集，再进行糖基化分析。唾液酸化糖肽作为糖肽中具有特殊结构的一类糖肽，同时具有亲水性、带电性。若采用常规糖肽富集方法，会导致低含量唾液酸糖肽产生选择性差、回收率低等问题，影响对唾液酸糖基化的进一步研究。因此需要发展具有高选择性的精准富集材料与方法。本项目以唾液酸糖肽为研究对象，结合其结构特性，发展以碱性氨基酸修饰的唾液酸糖肽精准富集材料，建立有特色的精准富集方法，解决低丰度唾液酸糖肽的富集难题。最后将方法应用于正常与肝癌病人血清酶解液中唾液酸糖肽的精准富集与鉴定中，实现全面、精准的唾液酸糖基化分析，为癌症发生发展等生命过程的研究提供方法基础和技术支持。

The sialylation of proteins plays significant roles in carcinoma processes and the sialylation research will help make the early diagnosis. All kinds of the glycopeptides are enriched by the routine research methods, while the sialic-acid glycopeptides with low abundance and amount cannot be enriched due to the matrix interference. So further research of sialylation cannot be achieved.Two characters of hydrophilicity and electriferous property of the sialic-acid glycopeptides give us the enlightenment. Based on the characters, basic amino acid modified accurate enrichment medium can be prepared. The specific accurate enrichment method can be built. At last, this method will be applied to the sialylation research in serum and the research foundation of carcinoma mechanism can be supported.