长期研究发现，降糖三黄片在改善2型糖尿病动物糖脂代谢、胰岛β细胞结构及功能等方面具有良好作用。本研究基于前期工作基础，进一步验证该药的作用机制。自噬又称II型程序性死亡，是真核生物常见的生命现象，与胰岛β细胞结构、功能密切相关。研究表明，糖脂毒性、内质网应激可以改变胰岛β细胞自噬活性，从而影响胰岛细胞的结构、功能。本研究首先在体外实验通过电镜观察及MTT、流式细胞检测以确认自噬参与高糖高脂毒性所致的胰岛β细胞损伤，且降糖三黄片对细胞活性具有保护作用；观察在该药干预下，细胞结构、自噬小体及相关基因、蛋白表达的改变，以验证该药参与自噬调控；继之应用自噬诱导、抑制剂与降糖三黄片分别在体外、体内实验进行干预，对比探讨降糖三黄片的自噬调控作用能否作为减轻2型糖尿病胰岛β细胞损伤的治疗手段；最后从内质网应激角度，进一步阐明降糖三黄片自噬调控的作用机制。本研究可望为中医药治疗2型糖尿病提供新的思路。

Long-term studies found that Jiangtang Sanhuang tablets has a good effect in improving glucose and lipid metabolism, pancreatic β-cell structure and function in type 2 diabetes animal. Based on the previous work, this study will further verify the mechanism of action of the drug. Autophagy, also known as type II programmed death, is a common phenomenon in eukaryotes, and related with pancreatic β-cell structure and function closely. Studies have shown that high glucose and high lipid toxicity, endoplasmic reticulum stress can change the pancreatic β-cell autophagic activity, thus affecting the islet cell structure and function. In this study, first in vitro experiments by electron microscopy and MTT, flow cytometry to confirm the autophagy involved in high glucose and high lipid toxicity induced pancreatic β-cell injury, and Jiangtang Sanhuang tablets have a protective effect on cell activity. According to Jiangtang Sanhuang tablets intervention, cell structure, autophagy and related genes, protein expression changes in order to verify the drug involved in autophagy regulation. Followed by autophagy induction, inhibitors and Jiangtang Sanhuang tablets were applied in vitro and in vivo experiments. To investigate the effect of Jiangtang Sanhuang tablets on autophagy regulation and its mechanism of action to alleviate pancreatic β-cell injury in type 2 diabetes mellitus. Finally, in the perspective of endoplasmic reticulum stress, the mechanism of Jiangtang Sanhuang tablets on autophagy regulation was further clarified. Meanwhile, this study is expected to provide new ideas for Chinese medicine treatment of type 2 diabetes.