15-PGDH是前列腺素生物降解的重要限速酶，申请人前期发现，15-PGDH基因过表达对胃癌细胞增殖、侵袭有明显抑制作用，但其分子机制并不清楚。课题组通过生物信息学软件预测了靶向15-PGDH的microRNA为miR-28-3P，且课题组前期工作发现MicroRNA‑28通过调控PTEN/PI3K/AKT信号通路促进胃癌的生长、侵袭和转移，所以我们的科学假说是miR-28-3P靶向15-PGDH调控 PTEN/PI3K/AKT信号通路，如果要证明这一科学假说成立，首先要证明15-PGDH与PTEN表达相关，于是我们检测了28例患者胃癌组织中15-PGDH及PTEN的相对表达量，并进行Spearman相关分析，结果显示15-PGDH与PTEN基因的表达呈正相关。所以本课题将对15-PGDH这一抑制胃癌的机制进行进一步探讨，这将为胃癌的靶向治疗提供新的思路。

15-hydroxyprostaglandin dehydrogenase (15-PGDH) is a rate-limiting enzyme ofprostaglandin. Our preliminary experiment shows that: The high expression of 15 -PGDH can inhibit the invasion and proliferation of gastric cancer, but itsmolecular mechanism is unclear. The target gene of miR-28-3P was predicted by bioinformatics analysis software, and 15 -PGDH is the target gene for miR-28-3P , and the previous work of the task group found that MicroRNA-28 promoted the growth, invasion, and transfer of gastric cancer by regulating the PTEN/PI 3K/AKT signal pathway., So our scientific hypothesis is that miR-28-3P targets 15 -PGDH regulating the PTEN/PI 3K/AKT signal path. If this scientific hypothesis is to be proved, we must proved that 15-PGDH is related to the expression of PTEN. So we tested the relative expression of 15-PGDH and PTEN in the tissue of 28 patients with gastric cancer and performed a Spearman correlation analysis. The results showed that 15-PGDH was positively correlated with the expression of PTEN gene. Therefore, this paper will further explore the mechanism of 15-PGDH inhibiting gastric cancer, which will provide new ideas for the target treatment of gastric cancer.