准好氧生物反应器填埋场N2O产生特性与机理研究

准好氧生物反应器填埋场因具有加速填埋场稳定化、降低渗滤液污染物浓度、减少甲烷气体排放及实现原位脱氮等技术环境优势而成为可持续填埋的前沿。但脱氮过程中微生物硝化与反硝化作用会导致温室气体氧化亚氮的产生，使其成为氧化亚氮的重要排放源。本项目拟采用准好氧生物反应器填埋场模拟实验装置，研究准好氧生物反应器填埋场垃圾稳定化过程中氧化亚氮的动态变化规律及影响氧化亚氮产生的关键因素；通过15N稳定同位素示踪技术研究氧化亚氮的产生途径；采用变性梯度凝胶电泳(PCR-DGGE)技术分析垃圾降解过程中微生物群落结构的变化及其与氧化亚氮变化之间的关系，初步揭示氧化亚氮的产生机理。项目研究成果将为准好氧生物反应器填埋场氧化亚氮的减排与控制提供理论基础和技术支撑。

The semi-aerobic bioreactor landfill has such technical and environmental advantages as accelerating landfill stabilization, reducing concentration of pollutants in leachate, emission reduction of methane, and in-situ removal of nitrogen, which made it the leading edge of sustainable landfill.But during the process of biological nitrogen removal, nitrification and denitrification lead to production of Nitrous Oxide, an important greenhouse gas, which makes the semi-aerobic bioreactor landfill an important emssion resource of greenhouse gas. An experimental device modeling semi-aerobic bioreactor landfill will be used in the project. The dynamic variation pattern of Nitrous Oxide produced in a semi-aerobic bioreactor landfill will be studied. The key factors that influence the porduction of Nitrous Oxide will be ascertained. Base on the 15N stable isotope trace method, the producing pathway of Nitrous Oxide will be ascertained.Based on the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technology,the community structure succession of the microorganism in the process of the stabalization of refuse and its relationship with Nitrous Oxide varation will be discussed to reveal the production mechnism of Nitrous Oxide. The research results can provide theory basis and technical supports for Nitrous Oxide emission reduction and control of semi-aerobic bioreactor landfill.