消癌解毒方是在国医大师周仲瑛教授的“癌毒”病机理论指导下配伍形成的。临床诊疗及药效实验结果均表明其具有确切的抗肿瘤效果和干预肿瘤炎性微环境作用，基于“癌毒”病机与炎性微环境的相似相通、关系密切，本项目结合文献研究及前期实验基础，拟通过体外药效干预实验，“植化分离”与“活性追踪”相结合，应用现代色谱波谱等分析技术，结合分子生物学、细胞生物学、表观遗传学等手段，分别采用消癌解毒方、复方组份及其部位、相关单体成分进行动态干预、筛选和验证，观察分析对以肿瘤细胞、免疫细胞及相关细胞因子为代表的肿瘤炎性微环境干预效应，追踪分离活性部位及单体，辅以计算机分子模拟技术，研究揭示复方干预炎性微环境的药效物质基础及分子机制，探讨复方配伍机理、完善癌毒病机理论，为肿瘤的临床诊疗以及复方的进一步开发利用提供依据。

Xiao’ai Jiedu Fang was prescribed under the guidance of "cancer pathogen" pathogenic theory put forward by Prof. Zhou Zhong-ying, one of the nation-level Chinese medicine masters. Clinical and experiment results all showed that it may have certain anti-tumor effect and intervene tumor inflammatory microenvironment. As the pathogenesis of "cancer pathogen" is similar and closely related to inflammatory microenvironment, this project is planned to perform in vitro efficacy interventional experiments based on literature research and the previous experiments, combined with "plant chemical separation" and "activity tracking". With the application of modern chromatography spectrum analysis technology, Xiao’ai Jiedu Fang solution, its composition and monomer components are dynamically intervened, screened and verified respectively. Combining techniques concerning molecular biology, cytobiology, and epigenetics, the experiments are aimed to observe and analyze the interventional efficacy of inflammatory microenvironment of tumors such as tumor cells, immune cells and related cytokines as well as to track the separated part and monomer. With the help of computer molecular simulation technology, this study is to reveal the pharmacodynamic material basis and molecular mechanism of intervened effect of compound prescriptions on inflammatory microenvironment and explore the compatibility mechanism to supplement cancer pathogenesis theory, for providing foundation for clinical diagnosis of cancer and further development and utilization of Xiao’ai Jiedu Fang.