基于结构光照明的数字全息显微研究

本项目旨在研究基于结构光照明的超分辨数字全息显微(DHM)方法。由空间光调制器(SLM)产生两种不同的结构照明光(条纹结构照明光和随机相位结构照明光)用于照明样品。利用光栅衍射和针孔滤波构建物参共路的点衍射DHM装置，克服环境扰动对DHM测量和成像结果的影响。条纹结构照明和随机相位结构照明通过"合成数值孔径"，可将DHM空间成像分辨率提高一倍。与传统结构照明显微方法相比,本方法除了可以对振幅型物体进行超分辨成像,还可以对相位型物体进行超分辨成像。同时,这两种结构照明光还可以实现DHM的自动调焦和纵向层析成像。该研究将为高性能、实用化数字全息显微镜的开发和应用打下良好的理论和技术基础。

This project aims at investigating the Digital Holographic Microscopy (DHM) with super-resolution and slicing imaging ability based on structured illumination. Two kinds of structured illuminations, i.e., fringe structured illumination and random-phase illumination, are generated by a spatial light modulator (SLM), and are used for the illumination of DHM. Commom-path DHM configuration is constructed by doubling the object wave via a diffraction grating and filtering one copy by a pinhole. Fringe strucuted illumination and random-phase illumiantions enable to enhance the spatial resolution of DHM by two folds via synthesizing a larger numerical aperture. Unlike the traditional structured illumination microscopy, which can only be used for amplitude specimen, the proposed method can be used for both amplitude and phase specimen. Besides,the fringe and random-phase structured illuminations will be investigated for autofocusing and slicing imaging of DHM. Above all, this research proposal will contribute significantly to developing a high-performance DHM and unlocking the potential of the application of the structured-illumination DHM.