胭脂鱼和“四大家鱼”等产漂流性卵鱼类具有重要的经济和物种价值，其鱼卵在随水迁移的同时进行孵化，而鱼卵生物属性和水流水力特性的分离导致了现有成果的局限性。本项目以山区紊动水体中漂流性鱼卵的异步迁移和胚体发育过程为研究对象，借助改进的粒子测速技术和自主开发的模拟鱼卵，解析紊动水体中漂流性鱼卵随水异步迁移的微观和宏观过程，分析其异步迁移规律和空间分布特性；研究紊动水体中流速和水温对鱼卵胚体孵化时间、孵化率、关键生理过程的影响，寻求鱼卵孵化的水流阈值条件；观测水流作用下卵膜的变形、细胞增殖和基因表达的特性，探索紊流水体中力和能量向卵核的传导过程和衰减规律，揭示紊动水体对鱼卵胚体发育过程的影响机理，并提出漂流性鱼卵随水迁移过程中的保护机制。通过本项目的研究，可深入认识山区河流紊动水体中漂流性鱼卵的运动过程及胚体发育的响应机制，具有重要的科学价值。

Drifting egg-laying fishes, such as the Chinese Sucker and the "Four Major Chinese Carps", have important economic and species values. The hatching process of these fish eggs is synchronized with their migration in water. But the separation of the eggs’ biological properties from fluid hydraulic properties has led to the limitations of available results. In present study, the embryonic development details of drifting fish eggs during their asynchronous migration in turbulent mountain rivers will be studied. Specially, the following main topics concerning to the moving and embryonic hatching of drifting fish eggs will be studied: (1) the micro and macro asynchronous migration mechanism and the spatial distribution will be analyzed by modified particle imaging velocimetry and self-developed artificial fish eggs; (2) in order to find the threshold hatching conditions, the effects of velocity and water temperature on their incubation time, hatching rate and key physiological processes will be investigated; (3) the deformation, chemical and physiology variation of zona pellucida, water hardened layer and egg nuclei under water shear stress will be observed; (4)The transfer of fluid force and energy from water to inner egg nuclei, cell proliferation rate and gene expression situation will be investigated. Based on that, the influence mechanisms of turbulent water on embryonic development of drifting fish eggs will be revealed, and the protection strategy would be also put forward. The studies of this proposal are expected to provide insight into the asynchronous migration mechanism and embryonic development of drifting fish eggs in response to turbulent fluid in mountain rivers, which is of great scientific value.