口腔鳞癌对化疗耐受是影响预后的重要原因，本课题组前期研究发现口腔鳞癌的化疗耐受与低氧微环境密切相关。而肿瘤微环境中除了低氧特征外还存在大量的肿瘤相关巨噬细胞（TAMs），且低氧微环境能通过肿瘤细胞募集更多的TAMs。我们猜想低氧微环境募集的TAMs是否也对口腔鳞癌进展及化疗耐受起到作用。本研究拟开展以下探索：（1）明确低氧下口腔鳞癌细胞对TAMs的趋化、极化作用，探索起到关键作用的细胞因子。（2）研究TAMs对口腔鳞癌顺铂耐受的影响，分析巨噬细胞炎性蛋白5(MIP-5)在其中的作用并探讨具体机制。（3）建立口腔鳞癌人源肿瘤组织移植模型，联合顺铂和MIP-5抑制剂，检测抑瘤作用。通过本研究将阐明低氧微环境下口腔鳞癌细胞与TAMs的相互作用关系及其在肿瘤的进展和化疗耐受中的作用。

Chemo-resistance is one of the most important prognostic factors in the treatment of oral squamous cell carcinoma.Our study found that chemo-resistance is closely related to the hypoxic microenvironment.In addition,there are a large number of tumor associated macrophages (TAMs) in tumor microenvironment, and the hypoxia microenvironment can raise TAMs. We hypothesized that the TAMs raised by hypoxic microenvironment may also play a role in chemotherapy tolerance in OSCC.This study intends to carry out the following studies:(1) Analyze the effect of OSCC cells on the chemotaxis and polarization of TAMs in hypoxia,and the role of key cytokines..(2) Analyze the effects of supernatant secreted by TAMs in hypoxia cisplatin resistance of OSCC,and role of macrophage inflammatory protein 5(MIP-5). (3) Establish a human tumor tissue transplantation model of OSCC, and using cisplatin and MIP-5 inhibitor to detect the anti-tumor effect. In this study, we will clarify how TAMs can interact with OSCC cells in hypoxic microenvironment to promote tumor progression and chemotherapy resistance.