基于生物滴滤的3D-BER催化转化疏水性恶臭界面传质强化及微生物学机制

生物技术处理疏水性恶臭污染物存在气体停留时间长和去除能力低的瓶颈问题，界面气液传质和生物膜活性共调控是增效恶臭处理的有效途径。本项目以二甲基二硫醚(DMDS)为典型的疏水性恶臭代表物；以自产生物表面活性剂微生物为接种菌源；以负载磁性电子介体的椰壳炭为粒子电极兼生物膜载体，构建基于生物滴滤技术原理的3D-BER。系统开展直流电场胁迫下，三维电极生物膜协同自产生物表面活性剂提高疏水性恶臭气液两相传质速率和生物膜活性的研究。明确动力学参数对3D-BER电化学-生物转化DMDS效果和途径的影响；阐明电场和磁性电子介体作用与微生物自产生物表面活性剂之间的关联特性；探讨优势种群时空动态变化、微生物代谢DMDS的电子传递和生物膜活性响应机制。本项目的顺利实施将有助于揭示疏水性恶臭在3D-BER内催化转化过程的界面传质强化和微生物学机制，也为生物法处理其它疏水性气态污染物提供有价值的理论与技术参考。

Long gas residence time and low elimination capacity are the bottleneck problems of biotechnology during the process of hydrophobic odorous pollutants treatment. Co-regulation of interfacial gas-liquid mass transfer and biofilm activity is an effective way to enhance the treatment efficiency of odor. In this project, 3D-BER will be constructed based on the technology principle of biotrickling filter with dimethyl disulfide (DMDS) as a representation of hydrophobic odorous pollutants, biosurfactant-producing microorganisms as inoculums, and coconut husk biochar loaded with magnetic electron mediator as three-dimensional electrode and packing material for microorganism immobilization. Under the direct current electric field stress, the enhancement of gas-liquid two-phase mass transfer rate of hydrophobic odor and biofilm activity by three-dimensional electrode biofilm and self-produced biosurfactant will be studied. The effects of kinetic parameters on the degradation performance and pathway of DMDS by electrochemistry and biotransformation in 3D-BER will be determined; the correlation characteristics between biosurfactant produced by microorganism and electric field, magnetic electron mediator will be clarified; and the spatiotemporal dynamic change of dominant population, the electron transport and response mechanism of biofilm activity in the process of microbial metabolism of DMDS will be discussed. The successful implementation of this project is beneficial to revealing the enhancement of interfacial mass transfer and the mechanism of Microbiology druing the catalytic conversion of hydrophobic odor in 3D-BER，and it will also provide valuable theoretical and technical reference for biological treatment of other hydrophobic gaseous pollutants.