申请者通过前期临床实验发现，柴朴汤缓解哮喘急性发作的有效率达到93.1%，但其机制尚不明确。在预实验中发现，柴朴汤能调控T-淋巴细胞相关蛋白（T-lymphocyte maturation associated protein ,MAL）的表达，并且MAL可能通过其囊泡转运功能参与哮喘的发展过程。因此提出以下科学假说：柴朴汤可上调MAL的表达，维持上皮细胞的正常形态和功能，调控顶端膜蛋白细胞粘附分子CADM1的转运和分泌，延缓肝郁型哮喘的炎症反应。本项目拟以MAL敲基因肝郁型哮喘小鼠和上皮细胞系为模型，采用柴朴汤进行处理,分别在整体动物水平研究柴朴汤对上皮细胞形态和功能的影响，从细胞水平探讨柴朴汤含药血清对气道上皮MAL的影响和分子机制，进而解析柴朴汤对肝郁型哮喘的作用机制是否与MAL调控顶端膜蛋白转运和分泌相关。该项目的完成可以进一步阐明柴朴汤治疗哮喘的作用及相关分子机制。

Applicants found that the effective rate of Chaipu Decocton in relieving acute asthma attack was 93.1% through preliminary clinical experiments, but its mechanism action was unknown. In preliminary experiments, it was found that Chaipu Decocton regulate the expression of T-lymphocyte maturation associated protein (MAL), and MAL may participate the development of asthma through its vesicle transport function. Therefore, the following scientific hypothesis is proposed: Chaipu Decocton can upregulate the expression of MAL, maintain the normal morphology and function of epithelial cells, regulate the transportation and secretion of the cell membrane-promoting cell adhesion molecule (CADM1), and delay the inflammatory response of liver-depression asthma. This project intends to use MAL knockout gene asthma mice and epithelial cell lines as models for treatment, study the effect of Chaipu Decocton on the morphology and function of epithelial cells at the anima level and study the effect and molecular mechanism of Chaipu Decocton containing serum on airway epithelial MAL from the cell level. The effect of Chaipu Decocton-containing serum on the airway epithelial MALs was studied from the cellular level, and the mechanism was analyzed to find whether the Chaipu Decocton's mechanism action on asthma was related to the transport and secretion of MAL-regulated apical membrane proteins. The completion of the project can further clarify the role of Chaipu Decocton in the treatment of liver-depression asthma and related molecular mechanisms.