基于TAMs/CXCL1/HSPC轴诱导乳腺癌预转移龛探讨“邪之所凑，其气必虚”的科学内涵及干预研究

肿瘤转移“种子与土壤”学说与中医理论“邪之所凑，其气必虚”异曲同工，预转移龛(Pre-metastatic niche, PMN)的发现客观证实两种理论的合理性，靶向PMN的干预策略将会引起治疗模式的变革。我们前期证实消癖方靶向TAMs/CXCL1通路抑制乳腺癌肺转移，而TAMs/CXCL1可促进乳腺癌PMN形成和体内外转移，其机制与HSPC (Hematopoietic stem/progenitor cell)动员紧密相关。由此设想消癖方可通过抑制TAMs/CXCL1/HSPC轴阻断乳腺癌PMN形成，从而抑制肺转移。本研究拟通过活体细胞转移示踪、骨髓移植、体内外基因重组、临床标本检测及靶标垂钓芯片等技术探讨TAMs/CXCL1/HSPC轴诱导乳腺癌PMN形成的生物意义及中医药防治机制，以此阐释“邪之所凑，其气必虚”的科学内涵，为中医药治未病提供科学依据。

Cancer metastasis is considered to be related with “seed and soil” theory, which is similar to Chinese Medicine old saying “The region where pathogenic factors invades must be Qi deficiency”. The discovery of pre-metastatic niche (PMN) provides scientific evidence for both theories, and strategies targeting PMN might bring revolution of cancer treatment. We previously demonstrated that XIAOPI formula could inhibit breast cancer metastasis via targeting TAMs/CXCL1, and TAMs/CXCL1 could promote PMN formation and metastasis both in vitro and in vivo, the mechanism of which is closely correlated with the activation of HSPCs (Hematopoietic stem/progenitor cell). It is hypothesized that XIAOPI formula inhibits breast cancer PMN formation via blocking TAMs/CXCL1/HSPC axis. Our study was designed to explore the biological and therapeutic significance of TAMs/CXCL1/HSPC axis in the process of PMN formation of breast cancer, and therefore provides scientific evidence for the Chinese Medicine theory “The region where pathogenic factors invades must be Qi deficiency”.