树突状细胞与天然淋巴样细胞相互作用对Th2分化的影响和机理研究

Ⅱ型天然淋巴样细胞（Innate Lymphoid Cells 2， ILC2s）是一群新发现的具有与Th2细胞功能相似的、能快速分泌Th2型细胞因子的天然免疫细胞。树突状细胞（DC）是功能最强的专职抗原递呈细胞，处于启动和调控Th2免疫反应的中心地位。ILC2s与DC之间是否存在分工与合作，共同影响Th2细胞的分化和发育，尚未见报道。我们以此为契机，以过敏原木瓜蛋白酶(papain)诱导的哮喘模型为工具，以ILC2s与DC相互作用，共同调控Th2分化的发生发展的机理为核心科学问题。以现代细胞免疫学和分子免疫学方法为核心技术，研究在papain诱导的Th2免疫反应中，ILC2s与DC的变化对Th2分化的影响及机制，以期阐明它们如何分工、合作，进而调控Th2免疫反应的作用及可能的机理，为Th2型免疫反应的发生机制提供理论基础和实验依据。

Innate Lymphoid Cells 2 (ILC2s) is a group of newly discovered innate immune cells, which have similar function of Th2 and could rapid secrete Th2-type cytokines.Dendritic cells (DC) are the most powerful professional antigen- presenting cells, and play a central role in the initiation and regulation of Th2 immune response. Whether there is division of labor and cooperation between DC and ILC2s in regulating differentiation and development of Th2 cells,which is remain poorly understood.We take this as an opportunity and use papain induced asthma model as a tool to study whether DC and ILC2s could jointly regulating Th2 differentiation, as the core scientific issues.Using modern immunology and molecular experimental techniques, the project aims to investigate the changes of DC and ILC2s affect Th2 differentiation in papain induced Th2 immune response,in order to clarify the division of labor, cooperation between DC and ILC2s in regulating Th2 immune response as well as its mechanisms.This study will provide a theoretical basis and experimental evidence of the occurrence of Th2-type immune response.