羊草（Leymus chinensis）是松嫩草地的建群物种，由于过度放牧和土地利用的变化，羊草草原面积大大减少。研究发现羊草能够在一定的盐碱和放牧压力下良好的生长，然而对羊草能适应双重压力（盐碱和放牧）的机制尚不清楚。本研究采用15N同位素示踪技术，高效液相色谱结合冻干技术以及野外原位根系扫描技术，综合运用生态学和生理学知识，同时开展野外原位和温室试验。主要研究不同盐碱和刈割条件下根茎及根系形态整合特征与小分子根糖的关系；肥料15N在母株和子株的分配利用策略与根茎小分子糖的关系；小分子糖信号与蛋白质和有机酸渗透调节的关系。目的在于阐明小分子根糖组分在细胞渗透调节和信号传导中的作用，从而揭示羊草根茎对盐碱胁迫和放牧压力的"整合"作用机理。预期成果可以丰富和完善植物对逆境环境的适应性理论，为草地放牧管理及盐碱化草地恢复提供一定的理论和实践依据。

Leymus chinensis is dominant species in Songnen grassland, due to overgrazing and land-use change, this species is greatly reduced. Field study has shown L. chinensis can grow well in a certain amount of saline-alkali soil under moderate grazing pressure. However, its adaptation mechanism to salt stress and grazing pressure is not clear. Using 15N isotope technique and high performance liquid chromatography with frozen dry technology, and in situ roots scanning technology, integrated ecology and physiology knowledge, field and greenhouse experiments are carried out. The following parameters will be determined: root forms (length, and diameter and configuration) and root turnover, 15N recovery of mother and children rhizomes, soluble sugar components of different plant parts (roots, and roots, and stem base, and stem and leaves). Main aim of this study is to validate root sugar role in osmotic regulation and signal transduction in root cells. Especially to test whether there is "rhizome integration between mother plant and children plant" of L. chinensis. This information will enrich the theory of L. chinensis to salt-alkali resistance and grazing tolerance to animal grazing, giving some suggestion for the rehabilitation of salt-alkali grassland.