鸭肠炎病毒(DEV)是威胁水禽养殖业健康发展的重要病原之一，但对DEV研究多在在病毒基因组学、蛋白质组学和免疫防控上。项目申请人在前期研究中，首次发现和鉴定了DEV核衣壳蛋白及其受体蛋白激酶C抑制蛋白(PKCI)，并开展了PKCI和PKC对DEV增殖作用的影响研究。PKC既是PKCI的作用靶点，又能影响核转录因子NF-kB活性。NF-kB信号通路是动物机体在转录水平上影响多种基因表达的重要途径，在细胞增殖、免疫应答和炎症反应等都能发挥至关重要的作用。目前，有关NF-kB信号通路对DEV增殖过程中的作用及其机制尚未清楚。因此，本项目拟在前期基础上，应用荧光定量PCR、免疫共沉淀、荧光共定位等技术和方法，分析DEV增殖过程中NF-kB信号传导途径及其关键信号分子，明确NF-kB信号途径调控DEV增殖过程的分子机制，以期为阐明DEV致病机理和开展DVE免疫防控奠定理论基础。

Duck enteritis virus (DEV) is one of the major pathogens threatening to the healthy development of waterfowl industry, but the studies on DEV is more concentrated on viral genomes and proteomes and immunological prevention. In the preliminary studies, we had first identified the nucleocapsid protein of DEV and its receptor as protein kinase C inhibitor (PKCI), and carried out research on the impact of PKCI and PKC to DEV proliferation. PKC is target of PKCI but also affect the activity of the nuclear transcription factor NF-kB. As we all know, NF-kB signaling pathway is an important mechanism to influence a variety of gene expression at the transcriptional level in animal body, which play a crucial roles on cell proliferation, immune response and inflammation, etc. At present, the function and mechanism of NF-kB signaling pathway in DEV proliferation is still unclear. Therefore, the objective of this study, following our previous studies, is to analyze the role of NF-kB signaling pathway and the key signal molecular during the proliferation of DEV, by methods of fluorescence quantitative PCR, immunoprecipitation and fluorescence colocalization, etc. These studies may explain the molecular mechanism of NF-kB signaling pathway in the proliferation of DEV and provide a theoretical basis on elucidation for the pathogenic mechanisms of DEV and development for immune prevention of DVE.