热袍菌(Thermotogales)是探讨生命进化和生物制氢的重要物种。近年来已证实部分热袍菌目物种也可在有氧环境中生长代谢，其中以Komostoga olearia TBF19.5.1T的对氧胁迫的耐受性最高，但是对其抵御氧胁迫的机制却鲜有报道。在比较基因组学分析的基础上，本研究以K.olearia TBF 19.5.1T 为研究对象，分析氧胁迫对菌株生长代谢的影响，从全基因组范围分析不同氧胁迫下菌株在转录水平的表达差异，通过Q-PCR和体外表达验证，筛选与O2消耗、ROS祛除、DNA保护相关的功能基因，探讨其响应氧胁迫的可能途径，从而为揭示热袍菌目响应氧胁迫的分子机制和选育耐氧工程菌株提供新的理论依据。

Thermotogales provides good models for generating new insights into the evolution of organism and hydrogen fermentation. It have demonstrated that Thermotogales is able to grow in presence of O2, and Kosmotoga olearia TBF 19.5.1T is the supreme aerotolerant member of Thermotogale. However, the mechanisms defending oxidative stress of Thermotogale which is significant for us to understand the adaptative evolution is known little. The responses to oxidative stress of K.olearia TBF 19.5.1T will be investigated. And the effect of oxygen on its metabolism will be analysed by physiological and biochemical tests. The changes in gene expression under different oxidative stress will be analysed in global genome by transcriptome analysis. The founctional genes invovle in elimination of O2/ROS and DNA protection against oxygen in K.olearia TBF 19.5.1T will be selected and identified by Q-PCR and expressions in vitro. The putative pathway for oxidative stress response in K.olearia will be proposed.The result of this project will provide a novel basis for molecular mechanism to defense oxidative stress in Thermotogales and construction of aerotolerant genetic strains.