本项目拟开展樱桃茎腐病病原菌寄生疫霉RxLR效应分子PpAvh241在植物细胞中功能及作用机制的研究。RxLR效应分子在病原菌与寄主互作过程中有非常重要的作用。申请者前期通过对寄生疫霉基因组进行大量生物信息学筛选和分析，得到了一批候选RxLR效应分子。进一步实验发现其中一个典型的RxLR效应分子PpAvh241定位在植物的细胞膜上，根据初步实验结果和生物信息学分析该效应分子在致病过程中有重要功能。我们拟利用寄生疫霉瞬时沉默系统、烟草-病毒载体瞬时过表达系统、寄生疫霉-寄主植物互作系统和酵母双杂交系统，分别在烟草上过表达该效应蛋白、沉默寄生疫霉PpAvh241基因得到沉默转化子进行表型分析、沉默转化子对烟草、拟南芥及寄主植物樱桃的致病力的变化分析、植物抗病相关基因表达检测及效应蛋白的互作靶蛋白的筛选等实验，最终确定PpAvh241在侵染过程中的功能及作用机制。

In this project, we will study on the function and signaling pathway of RxLR effector PpAvh241 of the pathogen Phytophthora parasitica of cherry stem rot. RxLR effectors played an important role during the interaction between pathogen and host. In this paper, a large number of RxLR candidate effectors were obtained by screening and analyzing in the early stage. Further experiments revealed that a typical RxLR effector candidate, PpAvh241, was localized on the cell membrane of the plants. It is presumed that PpAvh241 has an important toxic function during infection based on the preliminary experimental results and bioinformatics analysis. However, its function and mechanism need to be further explored. We propose using of P. parasitica transient silencing transformation system to gain the PpAvh241 gene silencing mutants and analyze their phenotypes, over expressing the protein on Nicotiana benthamiana to analyze the function and subcellular localization, testing the virulence of P. parasitica silencing mutants to host plants and the expression of genes related to disease resistance in plants, Screening of interacting proteins of the RxLR effector Avh241, finally confirm the function and mechanism of PpAvh241 during the host-pathogen interaction.