PHT1翻译后磷酸化修饰调控水稻磷养分吸收的分子机制研究

我国水稻生产过度依赖化肥投入，造成严重的环境污染。提升水稻对磷养分吸收的效率对水稻产量的可持续增加具有重要的战略意义。水稻磷养分吸收转运蛋白翻译后调控是当前国际上研究的前沿和热点。磷酸盐转运体蛋白PHT1的磷酸化和脱磷酸化对植物体内的Pi水平的动态平衡起着关键的调节作用，申请人相关最新成果发表于Plant Cell（2015）。PHT1的磷酸化水平除受CK2激酶调节外，本研究新近发现蛋白磷酸酶PP2C家族的OsPP95和OsPP46也与PHT1结合来调控水稻磷养分的吸收，推测可能参与PHT1磷酸化水平的负向调控。为进一步阐明PHT1在翻译后水平的磷酸化修饰调控机制，本研究采用前沿分子生物学和生物化学手段对相关基因的功能和作用模型进一步深入解析，为提高作物磷吸收利用效率提供理论依据。

Chemical fertilizer has been over applied on rice growing in China so that heavily threatening the environment. Enhancing Pi-uptake efficiency in rice has great significance for the sustainability of agriculture. Post-translation modification is the leading edge and hotspots of phosphate-uptake regulation in rice. The phosphate transporter proteins PHT1 are the key protein family for phosphate uptake and translocation. Phosphorylation and dephosphorylation of PHT1 is finely regulated for maintaining Pi homestasis in rice. The authors’ recent progress on this topic is published on Plant cell in 2015. Moreover, besides we find that phosphorylation of PHT1 is regulated by the protein kinase CK2, we rencently find protein phosphatase OsPP95 and OsPP46 may be involved in the modification of PHT1 activity. Not only direct binding is found between the phosphatase and PHT1, but also the two phosphatase regulate phosphate uptake as well. In order to further elucidate the detail of the relationship between the proteins, we apply this fund do more research on this field using modern molecular and biological technologies. It would provide more detail information for enhancing Pi- uptake efficiency in rice.