巴西橡胶树的乳管是合成和储存天然橡胶的场所，树干树皮中的次生乳管与天然橡胶产量密切相关，由维管形成层分化而来。茉莉酸(JA)是调节橡胶树乳管分化的关键信号分子。最近我们发现组蛋白去乙酰化酶(HDA)的抑制剂曲古抑菌素A(TSA)也能诱导乳管分化，而且HDA活性、尤其是HDA6含量和基因表达都受JA影响，推测HDA6可能作为负调控因子介导JA诱导乳管分化。利用本研究组建立的橡胶树乳管分化诱导实验系统，采用实验形态学、生化和分子生物学技术，鉴定与HDA6互作的JA信号途径三个关键环节COI1、JAZ和MYC的成员、分析JA对组蛋白乙酰化修饰程度和位点的影响并鉴定HDA6去乙酰化修饰位点、通过药理学和转基因技术鉴定HDA6在调控乳管分化方面的功能。研究结果对于阐明HDA介导JA诱导乳管分化的分子机制，加深认识形成层细胞分化机制和通过转基因改良橡胶树的产胶潜力具有重要的理论意义和实际应用价值。

The secondary laticifer are the site for the synthesis and storage of natural rubber in the bark of rubber tree，differentiated from vascular cambia. The number of the secondary laticifers in the trunk bark is positively correlated to the rubber yield. We have testified that the jasmonic acid (JA) was the key signal molecule to regulate the secondary laticifers differentiation. Recently, we found the effect of trichostatin A (TSA), an inhibitor of histone deacetylases (HDA), on inducing the secondary laticifer differentiation, and that the level of total histone acetylation, the content and the gene expression of HDA6 are affected by JA. Based on these findings, we suggest that HDA6 act as a negative regulator in the regulation of jasmonate-induced laticifer differentiation. By using experimental morphological and molecular techniques, this project focuses on identifying the member of COI1, JAZ and MYC in jasmonate signaling that can interact with HDA6, and analyzing the effect of JA on the level and sites of the histone acetylation, and identifying the deacetylation sites in histone that are catalyzed by HDA6, and the role of HDA6 in the secondary laticifer differentiation by pharmacological technique and transgenic technology. The results are essential for understanding the vascular cambia differentiation into the secondary phleom parenchyma cells and improving the rubber yield potential of rubber tree by transgenic breeding.