种子次生休眠在自然条件下是广泛存在的，它给农林业生产带来相当多的问题。目前已知高温、低氧、低水势等因素可诱导种子产生次生休眠，但这些因素诱导种子次生休眠的生理机制尚不清楚。本课题以前期研究中的发现——在高温条件下渗透调节处理能有效阻止水曲柳种子进入热休眠这一现象为着眼点，拟将渗透调节、激素处理与种子热休眠诱导结合起来开展研究。通过考察渗透调节处理阻止种子热休眠诱导过程中脱落酸（ABA）、赤霉素（GA）、细胞分裂素（BA）和乙烯等激素的作用，分析各类激素在诱导种子热休眠过程中的作用关系及其对渗透调节的应答机制，在此基础上结合相关生理指标的测定，分析胚乳和胚中哪些生理活动在热休眠诱导过程中对关键激素和渗透调节做出响应。旨在揭示种子热休眠诱导过程中哪些关键激素通过何种机制实现调控作用，并阐明渗透调节通过何种生理机制影响该调控过程。从而丰富种子次生休眠的调控理论与技术，科学地指导农林业生产实践。

The secondary seed dormancy is widely existed in the natural conditions, which brings a lot of problems to the agricultural and forestry production. At present, high temperature, low oxygen, low water potential and other factors can induce secondary dormancy, but the physiological mechanism of these factors inducing secondary dormancy is not clear. This subject based on previous research findings, the phenomenon that osmoconditioning under high temperature can effectively prevent Fraxinus mandshurica seed from entering thermodormancy, as the starting point. It is proposed to combine osmoconditioning, hormonal treatment and induction of seed thermodormancy. The effects of various hormones on the induction of seed thermodormancy and their responses to osmoconditioning were analyzed by investigating the effects of exogenous hormones such as abscisic acid, gibberellin, cytokinin and ethylene during osmoconditioning circumventing seed thermodormancy induction. On this basis, the physiological and biochemical indexes in the endosperm and embryo were analyzed to determine which physiological activities responded to the key hormone and osmoregulation during the induction of thermodormancy. The purpose of this study is to reveal which key hormones regulate the seed during the induction of thermodormancy and to elucidate the mechanism by which osmoconditioning affects the regulatory process. Thereby enriching seed secondary dormancy regulation theory and technology, scientifically guide agricultural and forestry production practices.