共生真菌殖入凋落物分解过程对微生物群落构建及其碳释放功能的影响

微生物群落结构被认为是凋落物分解过程的小尺度关键性决定因素，而群落构建历史则被认可为微生物群落结构最基本的决定因素。在群落构建历史事件中植物组织共生真菌由共生系统殖入腐生系统被认为可能影响了微生物群落构建并进一步引起其碳释放功能的改变，然而到目前为止仍然没有相关研究对这一复杂过程的机理进行探索。项目组在前期的研究中发现一些植物中的共生真菌定殖引起了微生物群落结构的变异并进一步引起了凋落物碳释放过程的变化，项目申请也正是基于这一发现，尝试探索该复杂过程的机理。项目主要采用共生真菌殖入历史构建模拟技术，从微生物群落结构、分解酶活性及其基因表达等方面的内容对不同优势植物种的凋落物分解过程进行剖析，以期发现共生真菌殖入分解过程对微生物群落结构及其凋落物碳释放功能影响的过程机理。这一机理的探索将有助于更深入揭示森林凋落物分解的微生物学机制，对于碳释放预测模型的完善也将提供重要的科学依据。

Microbial community structure is now considered a key determinant of litter decomposition in fine scale, while community assembly history is also believed as the most basic determinant of microbial community structure. Among historical events of community assembly, the entering of symbiotic fungi in plant tissue into saprophytic system is speculated to affect microbial community assembly, and further change carbon release functioning. However, so far, the mechanism on this process has not been still explored by the researches. In the former work, project group found, in some plants, colonization of litter decomposition by symbiotic fungi led to a variation of microbial community structure, and further changed the litter carbon release. Based on this finding, project group try to explore the mechanism of this complicated ecological process. In this project, the litter decomposition processes of different dominant plant species will be analyzed, through the field experiment of simulating assembly history of colonization of symbiotic fungi, in the way of microbial community structure, decomposition enzyme activity and their gene expression in order to find the mechanism on effects of colonization of litter decomposition by symbiotic fungi on microbial community assembly and corresponding carbon release functioning. The exploration of this mechanism will contribute to further uncover the microbial driving force of forest litter decomposition, and also will provide important scientific basis for perfecting the prediction mode of carbon release.