番茄果实花青素合成调控基因ATROVIOLACIUM候选基因的功能验证与分析

花青素属于类黄酮化合物，广泛的存在蔬菜和水果中。长期食用花青素有利于人们改善视力，降低心血管疾病、癌症的发病率。花青素也可以提高植物对生物胁迫和非生物胁迫的抵抗能力。在前期工作中，申请人已经完成了番茄果实花青素合成调控基因ATROVIOLACIUM（ATV）的精细定位，获得了ATV唯一的候选基因SlMYBATV，该基因编码一个R3-MYB转录因子。以此为基础，本项目拟通过转基因的方法验证ATV候选基因的功能，结合转录组分析和蛋白互作研究揭示ATV蛋白质参与花青素合成调控的分子机理。研究结果将有助于阐明番茄果实中花青素合成调控的分子机理，为番茄以及茄果类其他蔬菜作物遗传改良提供重要的基因资源，同时也为基因编辑高品质番茄育种改良提供理论基础。

Anthocyanins are naturally occurring pigments ubiquitously present in vegetable and fruit and, as such, part of the human diet. Owing to their biological activity, anthocyanins may offer protection against many diseases, including coronary heart diseases and cancer. In addition, anthocyanin can provide protection against biotic and abiotic stresses for plants. The applicant of this project has been studying the regulation of anthocyanin biosynthesis in tomato for many years. Recently, the applicant has already finished the fine mapping of ATROVIOLACIUM (ATV), a locus underlying anthocyanin biosynthesis in tomato fruit. Only one candidate gene for ATV was obtained, termed as SlMYBATV. SlMYBATV encodes an R3-MYB repressor. This project will (1) verify the biological function of SlMYBATV by gene editing system and ectopic expression; (2) figure out the molecular mechanism of ATV protein in the biological process of anthocyanin regulation. The results of this project will not only improve the regulation network of anthocyanin biosynthesis in solanaceous fruit vegetables, but also may ultimately be promising for further engineering of plant secondary metabolism.