弱视主要受损部位在视皮质，是影响儿童视觉发育的主要疾病之一。视觉剥夺后视皮层结构和功能重组的有效干预是弱视防治研究的重点。功能近红外光谱技术（fNIRs）基于神经血管耦合，为视觉可塑性研究提供了有效技术平台。结合前期工作基础，本项目围绕“敏感期内单眼视觉剥夺后视皮层发育障碍，视功能柱‘漂移’和重组是弱视发病关键机制”假说，从视觉关键功能区响应及其异常激活状态筛选入手，利用fNIRs检测单眼剥夺大鼠视皮层oxy-Hb、deoxy-Hb和total-Hb的变化，评估视觉关键区脑功能状态与加工过程，构建光学脑地形图（时间序列图谱、血流动力学图谱）和空间三维模拟图，揭示视觉异常激活脑区空间分布和模式，明确视功能柱“漂移”和重组是弱视发生的关键机制；并探讨针刺对单眼剥夺后视皮层激活区空间分布和功能重构的影响，从视功能柱“漂移”和重组角度阐明针灸干预弱视的脑功能机制，为弱视针灸效应和应用研究提供依据。

The mainly damaged area leaded by amblyopia,which is one of the common diseases seriously affecting the visual function of children, is located as the visual cortex.To effectively intervene the structural and functional reorganization of visual cortexafter visual deprivation is significant to the prevention and the clinical treatment of amblyopia. Functional near-infrared spectroscopy (fNIRs) is based on the principle of neurovascular coupling, and provides an effective technology platform for visual plasticity research. Combined with previous work basis, and from the perspective of visual key functional area response and its abnormal activation state screening, this project focused on the hypothesis that “Monocular visual deprivation in the sensitive period leads to the visual cortical development disorder, meanwhile visual function column 'drift' and recombination are the key mechanisms of amblyopia”. In this project, fNIRs technology was used to detect the changes of oxy-Hb、deoxy-Hb and total-Hb of visual cortex of rats after monocular deprivation to assess the visual key areas of brain function and processing. Optical brain mapping (the time sequence map, hemodynamic profiles) and spatial 3D simulation diagram were built to reveal the spatial distribution and patterns of visual abnormal activation of brain regions, and to clearly show that the visual function column "drift" and recombination is the key mechanisms for the occurrence of amblyopia. Meantime, effects of acupuncture on spatial distribution and functional remodeling of apical cortex activation area in monocular deprivation were discussed and the brain functional mechanism of acupuncture intervention in amblyopia was explained from the perspective of the visual function column "drift" and recombination to provide the evidence for the study of acupuncture’s effects on amblyopia and its clinical practice.