胆囊癌的恶性程度高，预后差。如何早期发现肿瘤以及研发辅助手术切除的新型治疗方法是改善患者预后的重要因素。纳米材料技术近期的迅速发展为肿瘤的诊疗策略提供了新的平台。其中磁性碳纳米管是载带靶向分子及基因的良好载体。在前期研究中我们已成功地合成了具有良好磁相应性及水溶液稳定性的磁性碳纳米管，同时我们筛选出了对胆囊癌的增殖起到关键作用的基因SPOCK1。在此基础上，我们拟通过静电吸附作用将SPOCK1-siRNA负载于磁性碳纳米管上，同时利用酰胺反应将SPOCK1单克隆抗体偶联于其上。制备出同时具备胆囊癌影像学诊断功能及基因治疗功能的“靶向纳米MRI造影剂/SPOCK1-siRNA复合体”。并通过一系列体内外的生物学实验及理化分析该材料的物理学表征、对胆囊癌病灶MRI识别的特异性和敏感性以及作为基因治疗载体对胆囊癌细胞增殖的抑制能力。为今后开发胆囊癌诊疗一体的新技术提供实验基础和理论依据。

Gallbladder is always assosiated with high degree of malignant and poor prognosis.How to early dectect the leision and develop a new therapy to assist surgical treatment is ctritical. The fast development of nano-technique has established a good condition for tumor diagnosis and treatment research. Carbon nano tube is a good candidate to carry targeted marker or gene. Durning pervious research we have successfully synthesis magnetic carbon nano tube with good magnetism and dispersibility in water. Meanwhile we found SPOCK1 as a oncogene that can regulat proliferation and migration in gallbladder cancer cells. Base on these foundations, we plan to conjugate SPOCK1-siRNA as well as SPOCK1-monoantibody to the surface of functionalized magnetic carbon anotubes to synthesis   nano probes with both imaging diagnosis and genetic treament fuction. Systematic studies wil show the novel targeted contrast agent exhibit enhanced MRI effects.and biological properties on the cell and animal levels．We hope his study will provide the experimental basis for future development of new techniques for the diagnosis and targeted therapy of gallbladder cancer.