天然橡胶是重要的战略物资和工业原料，然而，军民高性能胶品种培育处于世界空白。橡胶烃长度是影响天然胶性能的关键因素之一，最新体外实验结果显示，其受CPTL\CPT\REF等组成的橡胶转移酶复合体的调控，但是，尚未有体内实验证据解析此复合体的成员构成。本项目将在已有橡胶树高效体胚再生体系和基因枪技术的基础上，构建橡胶树CRISPR/Cas9-sgRNA RNP基因组编辑体系，并在此基础上，利用CRISPR/Cas9-sgRNA RNP精准编辑特性，筛选切割CPTL\CPT\REF基因家族成员功能域的特异双sgRNA，突变在胶乳中高表达的CPTL\CPT\REF家族成员，分析基因突变对编辑植株橡胶烃分子量的影响，解析橡胶转移酶复合体的成员构成。项目的成功实施，将为高性能橡胶基因工程品种培育提供靶基因，同时推动橡胶树精准育种技术创新。

Natural rubber is an important strategic material and industrial raw materials, however, rubber clones specified for producing military and civil high performance natural rubber are margin in the world. Rubber hydrocarbon length is one of the key factors affecting the performance of natural rubber, and the latest in vitro experimental results show that the CPTL\CPT\REF complex controls the length of rubber hydrocarbon. However, the in vivo experimental evidence is lacking, and members of this complex are not clear. Based on the efficient somatic embryo regeneration system and particle gun technology, this project will first establish the rubber tree CRISPR/Cas9-sgRNA RNP genome editing system, and then delete functional domains of members of CPTL\CPT\REF gene family by double sgRNA, finally, confirm composition of members of rubber transferase complex by analysis of molecular weight of rubber hydrocarbon in edited plants. The successful implementation of the project will provide the target genes for the cultivation of high performance rubber, and promote the innovation of precise breeding technology.