包虫病是我国西部牧区的严重的人畜共患病，研制有效的新型疫苗是防止包虫病流行主要措施之一。随着分子生物学技术的发展，表位疫苗成为分子疫苗的研究热点，本研究为预防和根治新疆细粒棘球蚴病，构建针对终末宿主犬的Eg多表位基因疫苗，并研究该疫苗的免疫保护性。方法是利用生物信息学和噬菌体展示技术筛选Eg抗原表位，将终末宿主抗原EgA31与EgG1Y162的优势T-B联合表位, 用基因重组技术构建成pcDNA3.1/egG1Y162-EgA31多价优势表位基因疫苗。同时免疫小鼠感染模型，检测特异性抗体效价、T淋巴细胞增殖反应、Th1/Th2型细胞、Th17/Treg细胞活性等各项指标，探讨多价优势表位基因疫苗的免疫特点和对宿主的免疫保护作用。在此基础上，进一步用细粒棘球绦虫免疫终末宿主犬，观察该疫苗的现场保护效果，评价其应用前景，为寻找控制包虫病的有效疫苗奠定基础。

Hydatid disease is a serious zoonotic disease in the western pastoral areas in China. The development of new and effective vaccine is one of the main measures to prevent echinococcosis popular. With the development of molecular biology techniques, epitope vaccine is research focus of the molecular vaccine. For the prevention and cure of Xinjiang Echinococcus disease,we build Eg multi-epitope genic vaccine for terminal host dogs, and study the vaccine immune protection. With the method of bioinformatics and phage display choose Eg epitopes . The T-B combined advantage epitopes of terminal host antigens EgA31 and EgG1Y162 are constructed to form pcDNA3.1/egG1Y162-EgA31 multiple antigen genic vaccine using recombinant technology. Immune mouse infection model, specific antibody titers, T lymphocyte proliferative, activity indicators of Th1 and Th2 cells, Th17/Treg cell are detected and explore the characteristics of multiple antigen genic vaccine and the host immune protective effect. On this basis, further immune terminal host dogs with Echinococcus tapeworm ,observed the site protective effect of the vaccine, to evaluate the application prospects, and to lay the foundation for looking for an effective vaccine to control echinococcosis.