马蹄莲对Pcc引起的软腐病抗性分子机制研究

彩色马蹄莲是世界重要的新兴花卉，但软腐果胶杆菌（Pectobacterium carotovora subsp. Carotovora，Pcc）引起的细菌性软腐病，对彩色马蹄莲生产造成了极大地的威胁，尚没有有效的防治方法。寄主的抗性机制是控制病害的重要依据，但目前有关马蹄莲软腐病抗性机制还远未明晰。本项目依托前期系统收集的高、中、低不同抗性的156个马蹄莲资源，以Pcc侵染对不同抗性马蹄莲生长和细胞学的影响、及不同抗性马蹄莲对侵染病原生长的影响为依据，鉴定不同马蹄莲资源的抗性；通过与EST-SSR、NBS Profiling分子标记进行关联分析挖掘软腐病抗性相关基因；并利用RACE克隆2-3个抗性相关重要基因全长后，利用病毒介导的基因沉默和/或遗传转化基因表达进行基因功能的分析与初步验证，解析马蹄莲对Pcc引起的软腐病抗性的分子机制，以期为科学防治病害和抗性新品种培育提供理论依据。

The colored Zantedeschia is a very important new flower. However, production of the colored Zantedeschia is affected severely with soft rot caused by Pectobacterium carotovora subsp. Carotovora (called as Erwinia carotovora subsp. Carotovora before). Among the conventional ways to control soft rot: biological control, chemical control and cultivation measures, none is enough efficient. The ways based on plant resistance mechanism will be more efficient and direct, but the resistance mechanism of Zantedeschia spp. to soft rot is unknown. The 156 genetics with different resistance were collected in the previuos work. In this study, the influence of the pathagen Pcc on plant cell constitutive and plant growth, and Pathogen propagation/infection in plant after inoculation will be used as the efficient envidence to evaluate the phenotype resistance in Zantedeschia. The genes involving in the plant resistance will be marked and selected by association analysis combined the molecular markers from EST-SSR, NBS Profiling, and the phenotype. The whole sequence of 2-3 key genes will cloned by RACE, and identified their functions via expressing and/or siliencing them in the plants initially. All data will be used to explore the molecular mechanism of defense and resistance to soft rot in Zantedeschia, which will be valuable for making efficient control ways to soft rot and breeding new varieties with high resistance in the future.