清胰汤干预MSCs在体内归巢与分化治疗胰源性肺损伤的机制研究

急性肺损伤 (ALI) 是重症急性胰腺炎 (SAP)早期死亡的主要原因，间充质干细胞（MSCs）因具有细胞修复与免疫调节作用而为此病的治疗开辟了新途径，但其在损伤肺组织内的归巢与分化率很低，无法达到满意的治疗效果。本研究通过揭示SDF-1/CXCR4和Wnt/β-catenin信号通路对MSCs归巢迁移与定向分化的调控机制，探索清胰汤对以上通路的干预靶点及对MSCs定植存活的保护作用与机制，建立以安全有效、易于推广的中药作为全新的干预因素，调控并促进MSCs向靶组织迁移、提高其定向分化率的新策略，从而达到有效修复重建损伤肺组织的结构与功能、降低死亡率并改善预后的目的；通过分析不同移植时期与途径对MSCs体内命运的影响及与治疗效果的相关性，筛选出最优化治疗方案，为中西医结合治疗此类重大难治疾病开辟新的思路与途径，也为中医药在干细胞与再生医学领域的应用奠定基础。

Acute lung injury (ALI) is the leading cause of death in early severe acute pancreatitis (SAP). Treatment with Mesenchymal stem cells (MSCs) has opened up a new avenues for the disease by cellular repair and immunomodulation, but it is unable to reach a satisfactory therapeutic potential because of the low homing and differentiation rate in damaged lung. In this study, we investigate mechanisms of regulation of SDF-1 / CXCR4 and Wnt / beta-catenin signaling pathway in MSCs homing and differentiation, explore Qingyitang's interventionist targets and protective mechanism on MSCs survival, establish a safe, effective and easy way to regulate and promote MSCs migration to target tissue and enhance their directed differentiation rate using traditional Chinese medicine as a new intervening factors, thereby achieving the purpose of effective repair and reconstruct damaged lungs, reduce mortality and improve prognosis. By analyzing the correlation between transplant way, time and MSCs fate as well as theraputic effeciency, we'll filter out the optimized treatment program and oped up the new ways and ideas to the treatment of such kind of serious and refractory diseases, also lay foundation for application of Chinese medicine in the field of stem cells and regenerative medicine.