产生高水平活性氧是动、植物细胞防御病原真菌侵染的重要机制。相应地，病原真菌在侵染过程中需启动一系列的抗氧化反应（即氧化胁迫反应），清除或抵抗活性氧的伤害。可见，氧化胁迫反应是病原真菌与寄主互作的重要环节，与毒力密切相关。目前，对病原真菌氧化胁迫反应的调控机制尚缺乏清晰的认识。课题组研究发现，一个穿膜蛋白Bbtmp1受Bbhog1信号途径的调控，负调节昆虫病原真菌球孢白僵菌氧化胁迫反应和毒力。该发现为研究Hog1途径调控病原真菌氧化胁迫反应提供了重要线索。本项目拟通过鉴定Bbtmp1基因启动子的氧化胁迫反应"应答"元件，采用酵母单杂交技术获取调控Bbtmp1的转录调控子并研究其生物学功能，进一步利用染色质免疫共沉淀（CHIP）分离鉴定转录调控子调控氧化胁迫反应的靶标基因。项目的开展有望在揭示白僵菌氧化胁迫反应的调控机制方面取得新的突破，并为利用基因工程改良菌株抗逆性和毒力提供新的理论依据。

It is one of the important mechanisms to producing high level of reactive oxygen species (ROS) for animal and plant cells to preventing fungal pathogen infection. Collectively, fungal pathogen has to mount a counteracting antioxidant response, called oxidative stress responses, to scavenge or degrade ROS for their survival within the host and disease establishment. Thus, the oxidative stress response is a vital process for fungal pathogen to counteract with the host immune system and is involved in virulence. However,the regulatory mechanism of the oxidative stress response in fungal pathogens is still unclear.We found that a transmembrane protein,Bbtmp1,controlled by the Bbhog1 MAPK signal pathway, negatively regulated oxidative stress response and virulence in the insect fungal pathogen Beauveria bassiana.The findings suggested a valuable cue to explore the mechanism of oxidative stress response regulated by Bbhog1 pathway in the fungal pathogen.In this project,we are going to identify the oxidative stress response element in the promoter of Bbtmp1 by reinroducing the gene containing different lengths of promoter into ?Bbtmp1 mutant and detecting the sensitivities of the complementation transformants to oxidative stress. If the sensitivity to oxidative stresses is not complemented to the wild-type extent by reintroducing Bbtmp1 containing a certain length of the promoter,the response element might be in the flanking sequence of the 5'-end of the promoter. Then the yeast one-hybrid strategy will be used to identify the transcription regulator of Bbtmp1 when the fungus is stressed by oxidation. Following charactreization of roles of the transcription regulator in the fungal pathogen,the target genes of the transcription regulator will be identified and characterized using chromatin immunoprecipitation (CHIP) method as well as bioinformatics analysis. One of our goals of the project is to expect to achieve a new breakthrough on understanding the regulatory mechanism of oxidative stress response regulated by Bbhog1 pathway in B. bassiana. Another goal is to hope to provide a theoretical basis for enhancement of oxidation resistance and virulence of the fungal agent using genetic engineering.